@article{qiuBioprintingNeuralSystems2020,
	title = {Bioprinting Neural Systems to Model Central Nervous System Diseases},
	volume = {30},
	rights = {© 2020 The Authors. Published by {WILEY}‐{VCH} Verlag {GmbH} \& Co. {KGaA}, Weinheim},
	issn = {1616-3028},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201910250},
	doi = {10.1002/adfm.201910250},
	abstract = {To date, pharmaceutical progresses in central nervous system ({CNS}) diseases are clearly hampered by the lack of suitable disease models. Indeed, animal models do not faithfully represent human neurodegenerative processes and human in vitro 2D cell culture systems cannot recapitulate the in vivo complexity of neural systems. The search for valuable models of neurodegenerative diseases has recently been revived by the addition of 3D culture that allows to re-create the in vivo microenvironment including the interactions among different neural cell types and the surrounding extracellular matrix ({ECM}) components. In this review, the new challenges in the field of {CNS} diseases in vitro 3D modeling are discussed, focusing on the implementation of bioprinting approaches enabling positional control on the generation of the 3D microenvironments. The focus is specifically on the choice of the optimal materials to simulate the {ECM} brain compartment and the biofabrication technologies needed to shape the cellular components within a microenvironment that significantly represents brain biochemical and biophysical parameters.},
	pages = {1910250},
	number = {44},
	journaltitle = {Advanced Functional Materials},
	author = {Qiu, Boning and Bessler, Nils and Figler, Kianti and Buchholz, Maj-Britt and Rios, Anne C. and Malda, Jos and Levato, Riccardo and Caiazzo, Massimiliano},
	urldate = {2021-01-08},
	date = {2020},
	langid = {english},
	note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/adfm.201910250},
	keywords = {3D culture, Parkinson's disease, biofabrication, disease modeling, hydrogels},
}

@article{levatoShapeFunctionNext2020,
	title = {From Shape to Function: The Next Step in Bioprinting},
	volume = {32},
	rights = {© 2020 The Authors. Published by {WILEY}‐{VCH} Verlag {GmbH} \& Co. {KGaA}, Weinheim},
	issn = {1521-4095},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201906423},
	doi = {10.1002/adma.201906423},
	shorttitle = {From Shape to Function},
	abstract = {In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell-laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.},
	pages = {1906423},
	number = {12},
	journaltitle = {Advanced Materials},
	author = {Levato, Riccardo and Jungst, Tomasz and Scheuring, Ruben G. and Blunk, Torsten and Groll, Juergen and Malda, Jos},
	urldate = {2021-01-08},
	date = {2020},
	langid = {english},
	note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/adma.201906423},
	keywords = {biofabrication, bioinks, biological function, regenerative medicine, tissue hierarchy},
}

@article{abbadessaBiofunctionalization3DPrinted2023,
	title = {Biofunctionalization of 3D printed collagen with bevacizumab-loaded microparticles targeting pathological angiogenesis},
	volume = {360},
	issn = {01683659},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0168365923004443},
	doi = {10.1016/j.jconrel.2023.07.017},
	pages = {747--758},
	journaltitle = {Journal of Controlled Release},
	shortjournal = {Journal of Controlled Release},
	author = {Abbadessa, Anna and Nuñez Bernal, Paulina and Buttitta, Giorgio and Ronca, Alfredo and D'Amora, Ugo and Zihlmann, Carla and Stiefel, Niklaus and Ambrosio, Luigi and Malda, Jos and Levato, Riccardo and Crecente-Campo, José and Alonso, María José},
	urldate = {2023-07-20},
	date = {2023-08},
	langid = {english},
}

@thesis{nunezbernalCraftingTissueComplexity2024,
	title = {Crafting Tissue Complexity: Pioneering Layerwise and Volumetric Biofabrication Strategies for Advanced In Vitro Models},
	isbn = {978-90-393-7622-5},
	url = {https://dspace.library.uu.nl/handle/1874/433677},
	doi = {10.33540/2116},
	shorttitle = {Crafting Tissue Complexity},
	abstract = {The global rise in life expectancy is accompanied by an increasing prevalence of chronic diseases, posing economic burdens and impacting patients' well-being.  This surge in disease incidence challenges the drug discovery pipeline, calling for the adaptation of its rules and regulations to reduce the ever-increasing failure rates of new drugs. The staggering growth of the pharmaceutical industry in the last decades, and the high expenditure required to bring new drugs to the market is set to become an unsustainable issue in the coming years. Recently, far more attention has been focused on the preclinical phase of the pipeline, where drug evaluation culminates in animal testing, the current gold standard to ensure drug safety and efficacy before human trials. A consensus on the need for more complex and predictive human disease models has emerged and become a priority for scientists and policy makers in the past decade. 

Biofabrication, an emerging technology-driven field, has gained prominence in biomedical research for developing advanced in vitro models. Biofabrication is “the automated generation of biologically functional products with structural organization from living cells, bioactive molecules, biomaterials, cell aggregates such as micro-tissues, or hybrid cell-material constructs, through Bioprinting or Bioassembly and subsequent tissue maturation processes”. The last decades have seen the rapid development new bioprinting modalities, opening the door to the development of architecturally complex in vitro platforms where multi-cellular and multi-material structures can be more easily created. Considering the need for more complex preclinical models to bridge the translational gap between 2D in vitro models, animal testing and clinical trials, the overarching aim of this thesis was “to develop new biofabrication approaches, encompassing 3D bioprinting technologies, powerful biological building blocks, and smart biomaterials, that facilitate the development of advanced human in vitro models with native tissue-like functionality”. 

This research has introduced significant advancements within the field of biofabrication for the generation of human in vitro models. Through a comprehensive exploration that tackled challenges related to fundamental bioprinting principles, the biological intricacies, and the biochemical and material property requirements of bioprinted structures to better recapitulate native tissues, the developments described here have expanded the toolkit of biofabrication approaches. By introducing novel technologies, like the pioneering, layerless volumetric bioprinting strategy, and synergizing new and existing printing approaches to harness their unique advantages, this thesis showcases a variety of functional bioprinted tissue models that enable the study of biological processes in vitro. The thorough exploration of volumetric bioprinting, distinguished by its scalability, unparalleled design freedom, compatibility with advanced biological tools, and a growing library of smart materials, charts new paths toward the creation of clinically-relevant testing platforms. The bioprinted, tissue-specific in vitro models presented here offer enhanced physiological accuracy and predictability and hold the potential to incorporate patient-specific elements for personalized medicine. The toolkit developed in here represents a significant stride in bridging the translational gap of tissue-engineered in vitro models, particularly in the context of preclinical testing. All in all, the evolving landscape of biofabricated in vitro models holds promise for innovative approaches in the future.},
	institution = {Utrecht University},
	type = {dr.},
	author = {Núñez Bernal, Paulina},
	urldate = {2024-12-04},
	date = {2024-01-10},
	langid = {english},
	note = {Pages: E17563220240206},
}

@article{grammensThreedimensionalBoneMorphology2024,
	title = {Three‐dimensional bone morphology is a risk factor for medial postmeniscectomy syndrome: A retrospective cohort study},
	volume = {11},
	issn = {2197-1153, 2197-1153},
	url = {https://esskajournals.onlinelibrary.wiley.com/doi/10.1002/jeo2.12090},
	doi = {10.1002/jeo2.12090},
	shorttitle = {Three‐dimensional bone morphology is a risk factor for medial postmeniscectomy syndrome},
	abstract = {Abstract
            
              Purpose
              The study aims to identify differences in tibiofemoral joint morphology between responders (R group, no pain) to arthroscopic partial medial meniscectomy ({APMM}) versus medial postmeniscectomy syndrome patients ({MPMS} group, recurrent pain at 2 years postmeniscectomy) in a clinically neutrally aligned patient population. The second aim was to build a morphology‐based predictive algorithm for response to treatment ({RTT}) in {APMM}.
            
            
              Methods
              Two patient groups were identified from a large multicentre database of meniscectomy patients at 2 years of follow‐up: the R group included 120 patients with a {KOOS} pain score {\textgreater} 75, and the {MPMS} group included 120 patients with a {KOOS} pain score ≤ 75. Statistical shape models ({SSMs}) of distal femur, proximal tibia and tibiofemoral joint were used to compare knee morphology. Finally, a predictive model was developed to predict {RTT}, with the {SSM}‐derived morphologic variables as predictors.
            
            
              Results
              No differences were found between the R and {MPMS} groups for patient age, sex, height, weight or cartilage status. Knees in the {MPMS} group were significantly smaller, had a wider femoral notch and a smaller medial femoral condyle. A morphology‐based predictive model was able to predict {MPMS} at 2 years follow‐up with a sensitivity of 74.9\% (95\% confidence interval [{CI}]: 74.4\%–75.4\%) and a specificity of 81.0\% (95\% {CI}: 80.6\%–81.5\%).
            
            
              Conclusion
              A smaller tibiofemoral joint, a wider intercondylar notch and smaller medial femoral condyle were observed shape variations related to medial postmeniscectomy syndrome. These promising results are a first step towards a knee morphology‐based clinical decision support tool for meniscus treatment.
            
            
              Study Design
              Case–control study.
            
            
              Level of Evidence
              Level {IIIb}.},
	pages = {e12090},
	number = {3},
	journaltitle = {Journal of Experimental Orthopaedics},
	shortjournal = {J. exp. orthop.},
	author = {Grammens, Jonas and Van Haver, Annemieke and Danckaers, Femke and Vuylsteke, Kristien and Sijbers, Jan and Mahluf, Lotem and Angele, Peter and Kon, Elizaveta and Verdonk, Peter and {MEFISTO WP1 Group}},
	urldate = {2024-12-04},
	date = {2024-07},
	langid = {english},
}

@article{conteOrthobiologicInjectionsTreating2023,
	title = {Orthobiologic injections for treating degenerative meniscus lesions: a matter of facts? Ten years of clinical experience in a systematic review},
	issn = {26672545},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S2667254523000069},
	doi = {10.1016/j.jcjp.2023.100104},
	shorttitle = {Orthobiologic injections for treating degenerative meniscus lesions},
	pages = {100104},
	journaltitle = {Journal of Cartilage \& Joint Preservation},
	shortjournal = {Journal of Cartilage \& Joint Preservation},
	author = {Conte, Pietro and Anzillotti, Giuseppe and Di Matteo, Berardo and Gallese, Alessandro and Vitale, Umberto and Marcacci, Maurilio and Kon, Elizaveta},
	urldate = {2023-04-03},
	date = {2023-01},
	langid = {english},
}

@article{bernalVolumetricBioprintingComplex2019,
	title = {Volumetric Bioprinting of Complex Living-Tissue Constructs within Seconds},
	volume = {31},
	rights = {© 2019 The Authors. Published by {WILEY}‐{VCH} Verlag {GmbH} \& Co. {KGaA}, Weinheim},
	issn = {1521-4095},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201904209},
	doi = {10.1002/adma.201904209},
	abstract = {Biofabrication technologies, including stereolithography and extrusion-based printing, are revolutionizing the creation of complex engineered tissues. The current paradigm in bioprinting relies on the additive layer-by-layer deposition and assembly of repetitive building blocks, typically cell-laden hydrogel fibers or voxels, single cells, or cellular aggregates. The scalability of these additive manufacturing technologies is limited by their printing velocity, as lengthy biofabrication processes impair cell functionality. Overcoming such limitations, the volumetric bioprinting of clinically relevant sized, anatomically shaped constructs, in a time frame ranging from seconds to tens of seconds is described. An optical-tomography-inspired printing approach, based on visible light projection, is developed to generate cell-laden tissue constructs with high viability ({\textgreater}85\%) from gelatin-based photoresponsive hydrogels. Free-form architectures, difficult to reproduce with conventional printing, are obtained, including anatomically correct trabecular bone models with embedded angiogenic sprouts and meniscal grafts. The latter undergoes maturation in vitro as the bioprinted chondroprogenitor cells synthesize neo-fibrocartilage matrix. Moreover, free-floating structures are generated, as demonstrated by printing functional hydrogel-based ball-and-cage fluidic valves. Volumetric bioprinting permits the creation of geometrically complex, centimeter-scale constructs at an unprecedented printing velocity, opening new avenues for upscaling the production of hydrogel-based constructs and for their application in tissue engineering, regenerative medicine, and soft robotics.},
	pages = {1904209},
	number = {42},
	journaltitle = {Advanced Materials},
	author = {Bernal, Paulina Nuñez and Delrot, Paul and Loterie, Damien and Li, Yang and Malda, Jos and Moser, Christophe and Levato, Riccardo},
	urldate = {2019-11-20},
	date = {2019},
	langid = {english},
	keywords = {biofabrication, bioinks, bioresins, cell encapsulation, photopolymers, tomographic laser prototyping},
}

@article{grammensSmallMedialFemoral2020,
	title = {Small medial femoral condyle morphotype is associated with medial compartment degeneration and distinct morphological characteristics: a comparative pilot study},
	issn = {1433-7347},
	url = {https://doi.org/10.1007/s00167-020-06218-8},
	doi = {10.1007/s00167-020-06218-8},
	shorttitle = {Small medial femoral condyle morphotype is associated with medial compartment degeneration and distinct morphological characteristics},
	abstract = {Early-onset degeneration of the knee is linked to genetics, overload, injury, and potentially, knee morphology. The purpose of this study is to explore the characteristics of the small medial femoral condyle, as a distinct knee morphotype, by means of a landmark-based three-dimensional (3D) analysis and statistical parametric mapping.},
	journaltitle = {Knee Surgery, Sports Traumatology, Arthroscopy},
	shortjournal = {Knee Surg Sports Traumatol Arthrosc},
	author = {Grammens, Jonas and Van Haver, Annemieke and Danckaers, Femke and Booth, Brian and Sijbers, Jan and Verdonk, Peter},
	urldate = {2020-09-02},
	date = {2020-08-14},
	langid = {english},
}

@article{limOneStepPhotoactivation2020,
	title = {One‐Step Photoactivation of a Dual‐Functionalized Bioink as Cell Carrier and Cartilage‐Binding Glue for Chondral Regeneration},
	volume = {9},
	issn = {2192-2640, 2192-2659},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.201901792},
	doi = {10.1002/adhm.201901792},
	pages = {1901792},
	number = {15},
	journaltitle = {Advanced Healthcare Materials},
	shortjournal = {Adv. Healthcare Mater.},
	author = {Lim, Khoon S. and Abinzano, Florencia and Bernal, Paulina Nuñez and Albillos Sanchez, Ane and Atienza‐Roca, Pau and Otto, Iris A. and Peiffer, Quentin C. and Matsusaki, Michiya and Woodfield, Tim B. F. and Malda, Jos and Levato, Riccardo},
	urldate = {2020-12-01},
	date = {2020-08},
	langid = {english},
}

@article{pattappaPhysioxiaExpandedBone2020,
	title = {Physioxia Expanded Bone Marrow Derived Mesenchymal Stem Cells Have Improved Cartilage Repair in an Early Osteoarthritic Focal Defect Model},
	volume = {9},
	rights = {http://creativecommons.org/licenses/by/3.0/},
	url = {https://www.mdpi.com/2079-7737/9/8/230},
	doi = {10.3390/biology9080230},
	abstract = {Focal early osteoarthritis ({OA}) or degenerative lesions account for 60\% of treated cartilage defects each year. The current cell-based regenerative treatments have an increased failure rate for treating degenerative lesions compared to traumatic defects. Mesenchymal stem cells ({MSCs}) are an alternative cell source for treating early {OA} defects, due to their greater chondrogenic potential, compared to early {OA} chondrocytes. Low oxygen tension or physioxia has been shown to enhance {MSC} chondrogenic matrix content and could improve functional outcomes of regenerative therapies. The present investigation sought to develop a focal early {OA} animal model to evaluate cartilage regeneration and hypothesized that physioxic {MSCs} improve in vivo cartilage repair in both, post-trauma and focal early {OA} defects. Using a rabbit model, a focal defect was created, that developed signs of focal early {OA} after six weeks. {MSCs} cultured under physioxia had significantly enhanced in vitro {MSC} chondrogenic {GAG} content under hyperoxia with or without the presence of interleukin-1\β ({IL}-1\β). In both post-traumatic and focal early {OA} defect models, physioxic {MSC} treatment demonstrated a significant improvement in cartilage repair score, compared to hyperoxic {MSCs} and respective control defects. Future investigations will seek to understand whether these results are replicated in large animal models and the underlying mechanisms involved in in vivo cartilage regeneration.},
	pages = {230},
	number = {8},
	journaltitle = {Biology},
	publisher = {Multidisciplinary Digital Publishing Institute},
	author = {Pattappa, Girish and Krueckel, Jonas and Schewior, Ruth and Franke, Dustin and Mench, Alexander and Koch, Matthias and Weber, Johannes and Lang, Siegmund and Pfeifer, Christian G. and Johnstone, Brian and Docheva, Denitsa and Alt, Volker and Angele, Peter and Zellner, Johannes},
	urldate = {2020-09-02},
	date = {2020-08},
	langid = {english},
	note = {Number: 8},
	keywords = {cartilage, chondrogenesis, disease\&, early osteoarthritis, hypoxia, mesenchymal stem cells, modeling},
}

@article{ummarinoTherapeuticManipulationMacrophages2020,
	title = {Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis},
	volume = {10},
	rights = {http://creativecommons.org/licenses/by/3.0/},
	url = {https://www.mdpi.com/2079-4991/10/8/1562},
	doi = {10.3390/nano10081562},
	abstract = {Osteoarthritis ({OA}) is the most common joint pathology causing severe pain and disability. Macrophages play a central role in the pathogenesis of {OA}. In the joint microenvironment, macrophages with an M1-like pro-inflammatory phenotype induce chronic inflammation and joint destruction, and they have been correlated with the development and progression of the disease, while the M2-like anti-inflammatory macrophages support the recovery of the disease, promoting tissue repair and the resolution of inflammation. Nowadays, the treatment of {OA} in the clinic relies on systemic and/or intra-articular administration of anti-inflammatory and pain relief drugs, as well as surgical interventions for the severe cases (i.e., meniscectomy). The disadvantages of the pharmacological therapy are related to the chronic nature of the disease, requiring prolonged treatments, and to the particular location of the pathology in joint tissues, which are separated anatomical compartments with difficult access for the drugs. To overcome these challenges, nanotechnological approaches have been investigated to improve the delivery of drugs toward macrophages into the diseased joint. This strategy may offer advantages by reducing off-target toxicities and improving long-term therapeutic efficacy. In this review, we describe the nanomaterial-based approaches designed so far to directly or indirectly manipulate macrophages for the treatment of osteoarthritis.},
	pages = {1562},
	number = {8},
	journaltitle = {Nanomaterials},
	publisher = {Multidisciplinary Digital Publishing Institute},
	author = {Ummarino, Aldo and Gambaro, Francesco Manlio and Kon, Elizaveta and Torres Andón, Fernando},
	urldate = {2020-09-02},
	date = {2020-08},
	langid = {english},
	note = {Number: 8},
	keywords = {anti-inflammatory, drug delivery, immune system, innate immunity, macrophage, nanomaterial, nanoparticle, osteoarthritis},
}

@article{angeleCellbasedTreatmentOptions2021,
	title = {Cell-based treatment options facilitate regeneration of cartilage, ligaments and meniscus in demanding conditions of the knee by a whole joint approach},
	issn = {0942-2056, 1433-7347},
	url = {https://link.springer.com/10.1007/s00167-021-06497-9},
	doi = {10.1007/s00167-021-06497-9},
	abstract = {Abstract
            
              Purpose
              This article provides an update on the current therapeutic options for cell-based regenerative treatment of the knee with a critical review of the present literature including a future perspective on the use of regenerative cell-based approaches. Special emphasis has been given on the requirement of a whole joint approach with treatment of comorbidities with aim of knee cartilage restoration, particularly in demanding conditions like early osteoarthritis.
            
            
              Methods
              This narrative review evaluates recent clinical data and published research articles on cell-based regenerative treatment options for cartilage and other structures around the knee
            
            
              Results
              Cell-based regenerative therapies for cartilage repair have become standard practice for the treatment of focal, traumatic chondral defects of the knee. Specifically, matrix-assisted autologous chondrocyte transplantation ({MACT}) shows satisfactory long-term results regarding radiological, histological and clinical outcome for treatment of large cartilage defects. Data show that regenerative treatment of the knee requires a whole joint approach by addressing all comorbidities including axis deviation, instability or meniscus pathologies. Further development of novel biomaterials and the discovery of alternative cell sources may facilitate the process of cell-based regenerative therapies for all knee structures becoming the gold standard in the future.
            
            
              Conclusion
              Overall, cell-based regenerative cartilage therapy of the knee has shown tremendous development over the last years and has become the standard of care for large and isolated chondral defects. It has shown success in the treatment of traumatic, osteochondral defects but also for degenerative cartilage lesions in the demanding condition of early {OA}. Future developments and alternative cell sources may help to facilitate cell-based regenerative treatment for all different structures around the knee by a whole joint approach.
            
            
              Level of evidence
              {IV}.},
	journaltitle = {Knee Surgery, Sports Traumatology, Arthroscopy},
	shortjournal = {Knee Surg Sports Traumatol Arthrosc},
	author = {Angele, Peter and Docheva, Denitsa and Pattappa, Girish and Zellner, Johannes},
	urldate = {2021-06-04},
	date = {2021-03-05},
	langid = {english},
}

@article{castilhoHydrogelBasedBioinksCell2021,
	title = {Hydrogel-Based Bioinks for Cell Electrowriting of Well-Organized Living Structures with Micrometer-Scale Resolution},
	issn = {1525-7797, 1526-4602},
	url = {https://pubs.acs.org/doi/10.1021/acs.biomac.0c01577},
	doi = {10.1021/acs.biomac.0c01577},
	pages = {acs.biomac.0c01577},
	journaltitle = {Biomacromolecules},
	shortjournal = {Biomacromolecules},
	author = {Castilho, Miguel and Levato, Riccardo and Bernal, Paulina Nunez and de Ruijter, Mylène and Sheng, Christina Y. and van Duijn, Joost and Piluso, Susanna and Ito, Keita and Malda, Jos},
	urldate = {2021-01-15},
	date = {2021-01-07},
	langid = {english},
}

@article{gambaroDrugDeliverySystems2021,
	title = {Drug Delivery Systems for the Treatment of Knee Osteoarthritis: A Systematic Review of In Vivo Studies},
	volume = {22},
	issn = {1422-0067},
	url = {https://www.mdpi.com/1422-0067/22/17/9137},
	doi = {10.3390/ijms22179137},
	shorttitle = {Drug Delivery Systems for the Treatment of Knee Osteoarthritis},
	abstract = {Many efforts have been made in the field of nanotechnology to improve the local and sustained release of drugs, which may be helpful to overcome the present limitations in the treatment of knee {OA}. Nano-/microparticles and/or hydrogels can be now engineered to improve the administration and intra-articular delivery of specific drugs, targeting molecular pathways and pathogenic mechanisms involved in {OA} progression and remission. In order to summarize the current state of this field, a systematic review of the literature was performed and 45 relevant studies were identified involving both animal models and humans. We found that polymeric nanoparticles loaded with anti-inflammatory drugs (i.e., dexamethasone or celecoxib) are the most frequently investigated drug delivery systems, followed by microparticles and hydrogels. In particular, the nanosystem most frequently used in preclinical research consists of {PLGA}-nanoparticles loaded with corticosteroids and non-steroidal anti-inflammatory drugs. Overall, improvement in histological features, reduction in joint inflammation, and improvement in clinical scores in patients were observed. The last advances in the field of nanotechnology could offer new opportunities to treat patients affected by knee {OA}, including those with previous meniscectomy. New smart drug delivery approaches, based on nanoparticles, microparticles, and hydrogels, may enhance the therapeutic potential of intra-articular agents by increasing the permanence of selected drugs inside the joint and better targeting specific receptors and tissues.},
	pages = {9137},
	number = {17},
	journaltitle = {International Journal of Molecular Sciences},
	shortjournal = {{IJMS}},
	author = {Gambaro, Francesco Manlio and Ummarino, Aldo and Torres Andón, Fernando and Ronzoni, Flavio and Di Matteo, Berardo and Kon, Elizaveta},
	urldate = {2022-03-17},
	date = {2021-08-24},
	langid = {english},
}

@article{korpershoekSelectionHighlyProliferative2021,
	title = {Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin: A Novel Approach in Meniscus Tissue Engineering},
	volume = {22},
	issn = {1422-0067},
	url = {https://www.mdpi.com/1422-0067/22/16/8614},
	doi = {10.3390/ijms22168614},
	shorttitle = {Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin},
	abstract = {Meniscus injuries can be highly debilitating and lead to knee osteoarthritis. Progenitor cells from the meniscus could be a superior cell type for meniscus repair and tissue-engineering. The purpose of this study is to characterize meniscus progenitor cells isolated by differential adhesion to fibronectin ({FN}-prog). Human osteoarthritic menisci were digested, and {FN}-prog were selected by differential adhesion to fibronectin. Multilineage differentiation, population doubling time, colony formation, and {MSC} surface markers were assessed in the {FN}-prog and the total meniscus population (Men). Colony formation was compared between outer and inner zone meniscus digest. Chondrogenic pellet cultures were performed for redifferentiation. {FN}-prog demonstrated multipotency. The outer zone {FN}-prog formed more colonies than the inner zone {FN}-prog. {FN}-prog displayed more colony formation and a higher proliferation rate than Men. {FN}-prog redifferentiated in pellet culture and mostly adhered to the {MSC} surface marker profile, except for {HLA}-{DR} receptor expression. This is the first study that demonstrates differential adhesion to fibronectin for the isolation of a progenitor-like population from the meniscus. The high proliferation rates and ability to form meniscus extracellular matrix upon redifferentiation, together with the broad availability of osteoarthritis meniscus tissue, make {FN}-prog a promising cell type for clinical translation in meniscus tissue-engineering.},
	pages = {8614},
	number = {16},
	journaltitle = {International Journal of Molecular Sciences},
	shortjournal = {{IJMS}},
	author = {Korpershoek, Jasmijn V. and Rikkers, Margot and de Windt, Tommy S. and Tryfonidou, Marianna A. and Saris, Daniel B. F. and Vonk, Lucienne A.},
	urldate = {2021-10-19},
	date = {2021-08-10},
	langid = {english},
}

@article{korpershoekPotentialMeltElectrowritten2021,
	title = {Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells},
	volume = {22},
	issn = {1422-0067},
	url = {https://www.mdpi.com/1422-0067/22/20/11200},
	doi = {10.3390/ijms222011200},
	abstract = {Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting ({MEW}) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling ({FDM}) and with the clinically used Collagen Meniscus Implants® ({CMI}®). The scaffolds were seeded with meniscus and mesenchymal stromal cells ({MSCs}) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in {MEW} scaffolds, the {CMI}®, and fibrin gel control, yet within the {FDM} scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The {MEW} scaffolds showed a higher Young’s modulus when compared to the {CMI}® scaffolds and a higher yield point compared to {FDM} scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with {MEW} using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.},
	pages = {11200},
	number = {20},
	journaltitle = {International Journal of Molecular Sciences},
	shortjournal = {{IJMS}},
	author = {Korpershoek, Jasmijn V. and Ruijter, Mylène de and Terhaard, Bastiaan F. and Hagmeijer, Michella H. and Saris, Daniël B.F. and Castilho, Miguel and Malda, Jos and Vonk, Lucienne A.},
	urldate = {2022-05-30},
	date = {2021-10-18},
	langid = {english},
}

@article{rikkersProgenitorCellsHealthy2021,
	title = {Progenitor Cells in Healthy and Osteoarthritic Human Cartilage Have Extensive Culture Expansion Capacity while Retaining Chondrogenic Properties},
	volume = {13},
	issn = {1947-6035, 1947-6043},
	url = {http://journals.sagepub.com/doi/10.1177/19476035211059600},
	doi = {10.1177/19476035211059600},
	abstract = {Objective
              Articular cartilage-derived progenitor cells ({ACPCs}) are a potential new cell source for cartilage repair. This study aims to characterize endogenous {ACPCs} from healthy and osteoarthritic ({OA}) cartilage, evaluate their potential for cartilage regeneration, and compare this to cartilage formation by chondrocytes.
            
            
              Design
              {ACPCs} were isolated from full-thickness healthy and {OA} human cartilage and separated from the total cell population by clonal growth after differential adhesion to fibronectin. {ACPCs} were characterized by growth kinetics, multilineage differentiation, and surface marker expression. Chondrogenic redifferentiation of {ACPCs} was compared with chondrocytes in pellet cultures. Pellets were assessed for cartilage-like matrix production by (immuno)histochemistry, quantitative analyses for glycosaminoglycans and {DNA} content, and expression of chondrogenic and hypertrophic genes.
            
            
              Results
              Healthy and {OA} {ACPCs} were successfully differentiated toward the adipogenic and chondrogenic lineage, but failed to produce calcified matrix when exposed to osteogenic induction media. Both {ACPC} populations met the criteria for cell surface marker expression of mesenchymal stromal cells ({MSCs}). Healthy {ACPCs} cultured in pellets deposited extracellular matrix containing proteoglycans and type {II} collagen, devoid of type I collagen. Gene expression of hypertrophic marker type X collagen was lower in healthy {ACPC} pellets compared with {OA} pellets.
            
            
              Conclusions
              This study provides further insight into the {ACPC} population in healthy and {OA} human articular cartilage. {ACPCs} show similarities to {MSCs}, yet do not produce calcified matrix under well-established osteogenic culture conditions. Due to extensive proliferative potential and chondrogenic capacity, {ACPCs} show potential for cartilage regeneration and possibly for clinical application, as a promising alternative to {MSCs} or chondrocytes.},
	pages = {129S--142S},
	number = {2},
	journaltitle = {{CARTILAGE}},
	shortjournal = {{CARTILAGE}},
	author = {Rikkers, M. and Korpershoek, J.V. and Levato, R. and Malda, J. and Vonk, L.A.},
	urldate = {2022-03-17},
	date = {2021-12},
	langid = {english},
}

@article{veronesiBiosyntheticScaffoldsPartial2021,
	title = {Biosynthetic scaffolds for partial meniscal loss: A systematic review from animal models to clinical practice},
	volume = {6},
	issn = {2452199X},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S2452199X21001444},
	doi = {10.1016/j.bioactmat.2021.03.033},
	shorttitle = {Biosynthetic scaffolds for partial meniscal loss},
	pages = {3782--3800},
	number = {11},
	journaltitle = {Bioactive Materials},
	shortjournal = {Bioactive Materials},
	author = {Veronesi, F. and Di Matteo, B. and Vitale, N.D. and Filardo, G. and Visani, A. and Kon, E. and Fini, M.},
	urldate = {2021-06-04},
	date = {2021-11},
	langid = {english},
}

@article{zhangSingleCellBioprinting2021,
	title = {Single Cell Bioprinting with Ultrashort Laser Pulses},
	volume = {31},
	issn = {1616-301X, 1616-3028},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/adfm.202100066},
	doi = {10.1002/adfm.202100066},
	pages = {2100066},
	number = {19},
	journaltitle = {Advanced Functional Materials},
	shortjournal = {Adv. Funct. Mater.},
	author = {Zhang, Jun and Byers, Patrick and Erben, Amelie and Frank, Christine and Schulte‐Spechtel, Levin and Heymann, Michael and Docheva, Denitsa and Huber, Heinz P. and Sudhop, Stefanie and Clausen‐Schaumann, Hauke},
	urldate = {2022-03-17},
	date = {2021-05},
	langid = {english},
}

@article{zhangExtendingSingleCell2021,
	title = {Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations},
	volume = {12},
	issn = {2072-666X},
	url = {https://www.mdpi.com/2072-666X/12/10/1172},
	doi = {10.3390/mi12101172},
	abstract = {Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rate after transfer above 95\% through the entire pulse duration range. More cost efficient and compact laser sources with pulse durations in the picosecond range could be used for film-free bioprinting and single-cell transfer.},
	pages = {1172},
	number = {10},
	journaltitle = {Micromachines},
	shortjournal = {Micromachines},
	author = {Zhang, Jun and Geiger, Yasemin and Sotier, Florian and Djordjevic, Sasa and Docheva, Denitsa and Sudhop, Stefanie and Clausen-Schaumann, Hauke and Huber, Heinz P.},
	urldate = {2022-03-17},
	date = {2021-09-29},
	langid = {english},
}

@article{pattappaFibronectinAdherentCell2022,
	title = {Fibronectin Adherent Cell Populations Derived From Avascular and Vascular Regions of the Meniscus Have Enhanced Clonogenicity and Differentiation Potential Under Physioxia},
	volume = {9},
	issn = {2296-4185},
	url = {https://www.frontiersin.org/articles/10.3389/fbioe.2021.789621/full},
	doi = {10.3389/fbioe.2021.789621},
	abstract = {The meniscus is composed of an avascular inner region and vascular outer region. The vascular region has been shown to contain a progenitor population with multilineage differentiation capacity. Strategies facilitating the isolation and propagation of these progenitors can be used to develop cell-based meniscal therapies. Differential adhesion to fibronectin has been used to isolate progenitor populations from cartilage, while low oxygen or physioxia (2\% oxygen) enhances the meniscal phenotype. This study aimed to isolate progenitor populations from the avascular and vascular meniscus using differential fibronectin adherence and examine their clonogenicity and differentiation potential under hyperoxia (20\% oxygen) and physioxia (2\% oxygen). Human vascular and avascular meniscus cells were seeded onto fibronectin-coated dishes for a short period and monitored for colony formation under either hyperoxia or physioxia. Non-fibronectin adherent meniscus cells were also expanded under both oxygen tension. Individual fibronectin adherent colonies were isolated and further expanded, until approximately ten population doublings (passage 3), whereby they underwent chondrogenic, osteogenic, and adipogenic differentiation. Physioxia enhances clonogenicity of vascular and avascular meniscus cells on plastic or fibronectin-coated plates. Combined differential fibronectin adhesion and physioxia isolated a progenitor population from both meniscus regions with trilineage differentiation potential compared to equivalent hyperoxia progenitors. Physioxia isolated progenitors had a significantly enhanced meniscus matrix content without the presence of collagen X. These results demonstrate that combined physioxia and fibronectin adherence can isolate and propagate a meniscus progenitor population that can potentially be used to treat meniscal tears or defects.},
	pages = {789621},
	journaltitle = {Frontiers in Bioengineering and Biotechnology},
	shortjournal = {Front. Bioeng. Biotechnol.},
	author = {Pattappa, Girish and Reischl, Franziska and Jahns, Judith and Schewior, Ruth and Lang, Siegmund and Zellner, Johannes and Johnstone, Brian and Docheva, Denitsa and Angele, Peter},
	urldate = {2022-02-04},
	date = {2022-01-28},
}

@article{rikkersClinicalPotentialArticular2022,
	title = {The clinical potential of articular cartilage-derived progenitor cells: a systematic review},
	volume = {7},
	issn = {2057-3995},
	url = {https://www.nature.com/articles/s41536-021-00203-6},
	doi = {10.1038/s41536-021-00203-6},
	shorttitle = {The clinical potential of articular cartilage-derived progenitor cells},
	abstract = {Abstract
            Over the past two decades, evidence has emerged for the existence of a distinct population of endogenous progenitor cells in adult articular cartilage, predominantly referred to as articular cartilage-derived progenitor cells ({ACPCs}). This progenitor population can be isolated from articular cartilage of a broad range of species, including human, equine, and bovine cartilage. In vitro, {ACPCs} possess mesenchymal stromal cell ({MSC})-like characteristics, such as colony forming potential, extensive proliferation, and multilineage potential. Contrary to bone marrow-derived {MSCs}, {ACPCs} exhibit no signs of hypertrophic differentiation and therefore hold potential for cartilage repair. As no unique cell marker or marker set has been established to specifically identify {ACPCs}, isolation and characterization protocols vary greatly. This systematic review summarizes the state-of-the-art research on this promising cell type for use in cartilage repair therapies. It provides an overview of the available literature on endogenous progenitor cells in adult articular cartilage and specifically compares identification of these cell populations in healthy and osteoarthritic ({OA}) cartilage, isolation procedures, in vitro characterization, and advantages over other cell types used for cartilage repair. The methods for the systematic review were prospectively registered in {PROSPERO} ({CRD}42020184775).},
	pages = {2},
	number = {1},
	journaltitle = {npj Regenerative Medicine},
	shortjournal = {npj Regen Med},
	author = {Rikkers, Margot and Korpershoek, Jasmijn V. and Levato, Riccardo and Malda, Jos and Vonk, Lucienne A.},
	urldate = {2023-10-20},
	date = {2022-01-10},
	langid = {english},
}

@article{ummarinoVitroModelOsteoarthritis2023,
	title = {An \textit{in vitro} model for osteoarthritis using long‐cultured inflammatory human macrophages repeatedly stimulated with {TLR} agonists},
	issn = {0014-2980, 1521-4141},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/eji.202350507},
	doi = {10.1002/eji.202350507},
	abstract = {Abstract
            
              Osteoarthritis ({OA}) is characterized by an abundance of inflammatory M1‐like macrophages damaging local tissues. The search for new potential drugs for {OA} suffers from the lack of appropriate methods of long‐lasting inflammation. Here we developed and characterized an
              in vitro
              protocol of long‐lasting culture of primary human monocyte‐derived macrophages differentiated with a combination of M‐{CSF}+{GM}‐{CSF} that optimally supported long‐cultured macrophages ({LC}‐Mϕs) for up to 15 days, unlike their single use. Macrophages repeatedly stimulated for 15 days with the {TLR}2 ligand Pam3CSK4 ({LCS}‐Mϕs), showed sustained levels over time of {IL}‐6, {CCL}2, and {CXCL}8, inflammatory mediators that were also detected in the synovial fluids of {OA} patients. Furthermore, macrophages isolated from the synovia of two {OA} patients showed an expression profile of inflammation‐related genes similar to that of {LCS}‐Mϕs, validating our protocol as a model of chronically activated inflammatory macrophages. Next, to confirm that these {LCS}‐Mϕs could be modulated by anti‐inflammatory compounds, we employed dexamethasone and/or celecoxib, two drugs widely used in {OA} treatment, that significantly inhibited the production of inflammatory mediators. This easy‐to‐use
              in vitro
              protocol of long‐lasting inflammation with primary human macrophages could be useful for the screening of new compounds to improve the therapy of inflammatory disorders.},
	pages = {2350507},
	journaltitle = {European Journal of Immunology},
	shortjournal = {Eur J Immunol},
	author = {Ummarino, Aldo and Pensado‐López, Alba and Migliore, Roberta and Alcaide‐Ruggiero, Lourdes and Calà, Nicholas and Caputo, Michele and Gambaro, Francesco M. and Anfray, Clément and Ronzoni, Flavio L. and Kon, Elizaveta and Allavena, Paola and Torres Andón, Fernando},
	urldate = {2023-10-10},
	date = {2023-10-06},
	langid = {english},
}

@article{terpstraBioinkCartilagederivedExtracellular2022,
	title = {Bioink with cartilage-derived extracellular matrix microfibers enables spatial control of vascular capillary formation in bioprinted constructs},
	volume = {14},
	issn = {1758-5082, 1758-5090},
	url = {https://iopscience.iop.org/article/10.1088/1758-5090/ac6282},
	doi = {10.1088/1758-5090/ac6282},
	abstract = {Abstract
            
              Microvasculature is essential for the exchange of gas and nutrient for most tissues in our body. Some tissue structures such as the meniscus presents spatially confined blood vessels adjacent to non-vascularized regions. In biofabrication, mimicking the spatial distribution of such vascular components is paramount, as capillary ingrowth into non-vascularized tissues can lead to tissue matrix alterations and subsequent pathology. Multi-material three-dimensional (3D) bioprinting strategies have the potential to resolve anisotropic tissue features, although building complex constructs comprising stable vascularized and non-vascularized regions remains a major challenge to date. In this study, we developed endothelial cell-laden pro- and anti-angiogenic bioinks, supplemented with bioactive matrix-derived microfibers ({MFs}) that were created from type I collagen sponges (col-1) and cartilage decellularized extracellular matrix ({CdECM}), respectively. Human umbilical vein endothelial cell ({HUVEC})-driven capillary networks started to form 2 d after bioprinting. Supplementing cartilage-derived {MFs} to endothelial-cell laden bioinks reduced the total length of neo-microvessels by 29\%, and the number of microvessel junctions by 37\% after 14 d, compared to bioinks with pro-angiogenic col-1 {MFs}. As a proof of concept, the bioinks were bioprinted into an anatomical meniscus shape with a biomimetic vascularized outer and non-vascularized inner region, using a gellan gum microgel suspension bath. These 3D meniscus-like constructs were cultured up to 14 d, with in the outer zone the {HUVEC}-, mural cell-, and col-1 {MF}-laden pro-angiogenic bioink, and in the inner zone a meniscus progenitor cell ({MPC})- and {CdECM} {MF}-laden anti-angiogenic bioink, revealing successful spatial confinement of the nascent vascular network only in the outer zone. Further, to co-facilitate both microvessel formation and {MPC}-derived matrix formation, we formulated cell culture medium conditions with a temporal switch. Overall, this study provides a new strategy that could be applied to develop zonal biomimetic meniscal constructs. Moreover, the use of {ECM}-derived {MFs} to promote or inhibit capillary networks opens new possibilities for the biofabrication of tissues with anisotropic microvascular distribution. These have potential for many applications including
              in vitro
              models of vascular-to-avascular tissue interfaces, cancer progression, and for testing anti-angiogenic therapies.},
	pages = {034104},
	number = {3},
	journaltitle = {Biofabrication},
	shortjournal = {Biofabrication},
	author = {Terpstra, Margo L and Li, Jinyu and Mensinga, Anneloes and de Ruijter, Mylène and van Rijen, Mattie H P and Androulidakis, Charalampos and Galiotis, Costas and Papantoniou, Ioannis and Matsusaki, Michiya and Malda, Jos and Levato, Riccardo},
	urldate = {2022-05-30},
	date = {2022-07-01},
}

@article{roncaDevelopmentHighlyConcentrated2023,
	title = {Development of a highly concentrated collagen ink for the creation of a 3D printed meniscus},
	volume = {9},
	issn = {24058440},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S240584402310315X},
	doi = {10.1016/j.heliyon.2023.e23107},
	pages = {e23107},
	number = {12},
	journaltitle = {Heliyon},
	shortjournal = {Heliyon},
	author = {Ronca, Alfredo and D'Amora, Ugo and Capuana, Elisa and Zihlmann, Carla and Stiefel, Niklaus and Pattappa, Girish and Schewior, Ruth and Docheva, Denitsa and Angele, Peter and Ambrosio, Luigi},
	urldate = {2024-01-18},
	date = {2023-12},
	langid = {english},
}

@article{grammensAutomatedLandmarkAnnotation2024,
	title = {Automated Landmark Annotation for Morphometric Analysis of Distal Femur and Proximal Tibia},
	volume = {10},
	rights = {https://creativecommons.org/licenses/by/4.0/},
	issn = {2313-433X},
	url = {https://www.mdpi.com/2313-433X/10/4/90},
	doi = {10.3390/jimaging10040090},
	abstract = {Manual anatomical landmarking for morphometric knee bone characterization in orthopedics is highly time-consuming and shows high operator variability. Therefore, automation could be a substantial improvement for diagnostics and personalized treatments relying on landmark-based methods. Applications include implant sizing and planning, meniscal allograft sizing, and morphological risk factor assessment. For twenty {MRI}-based 3D bone and cartilage models, anatomical landmarks were manually applied by three experts, and morphometric measurements for 3D characterization of the distal femur and proximal tibia were calculated from all observations. One expert performed the landmark annotations three times. Intra- and inter-observer variations were assessed for landmark position and measurements. The mean of the three expert annotations served as the ground truth. Next, automated landmark annotation was performed by elastic deformation of a template shape, followed by landmark optimization at extreme positions (highest/lowest/most medial/lateral point). The results of our automated annotation method were compared with ground truth, and percentages of landmarks and measurements adhering to different tolerances were calculated. Reliability was evaluated by the intraclass correlation coefficient ({ICC}). For the manual annotations, the inter-observer absolute difference was 1.53 ± 1.22 mm (mean ± {SD}) for the landmark positions and 0.56 ± 0.55 mm (mean ± {SD}) for the morphometric measurements. Automated versus manual landmark extraction differed by an average of 2.05 mm. The automated measurements demonstrated an absolute difference of 0.78 ± 0.60 mm (mean ± {SD}) from their manual counterparts. Overall, 92\% of the automated landmarks were within 4 mm of the expert mean position, and 95\% of all morphometric measurements were within 2 mm of the expert mean measurements. The {ICC} (manual versus automated) for automated morphometric measurements was between 0.926 and 1. Manual annotations required on average 18 min of operator interaction time, while automated annotations only needed 7 min of operator-independent computing time. Considering the time consumption and variability among observers, there is a clear need for a more efficient, standardized, and operator-independent algorithm. Our automated method demonstrated excellent accuracy and reliability for landmark positioning and morphometric measurements. Above all, this automated method will lead to a faster, scalable, and operator-independent morphometric analysis of the knee.},
	pages = {90},
	number = {4},
	journaltitle = {Journal of Imaging},
	shortjournal = {J. Imaging},
	author = {Grammens, Jonas and Van Haver, Annemieke and Lumban-Gaol, Imelda and Danckaers, Femke and Verdonk, Peter and Sijbers, Jan},
	urldate = {2024-05-13},
	date = {2024-04-11},
	langid = {english},
}

@article{pensado-lopezSyntheticPeptidesIL1Ra2024,
	title = {Synthetic peptides of {IL}-1Ra and {HSP}70 have anti-inflammatory activity on human primary monocytes and macrophages: Potential treatments for inflammatory diseases},
	volume = {55},
	issn = {15499634},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S1549963423000709},
	doi = {10.1016/j.nano.2023.102719},
	shorttitle = {Synthetic peptides of {IL}-1Ra and {HSP}70 have anti-inflammatory activity on human primary monocytes and macrophages},
	pages = {102719},
	journaltitle = {Nanomedicine: Nanotechnology, Biology and Medicine},
	shortjournal = {Nanomedicine: Nanotechnology, Biology and Medicine},
	author = {Pensado-López, Alba and Ummarino, Aldo and Khan, Sophia and Guildford, Anna and Allan, Iain U. and Santin, Matteo and Chevallier, Nathalie and Varaillon, Elina and Kon, Elizaveta and Allavena, Paola and Torres Andón, Fernando},
	urldate = {2024-05-06},
	date = {2024-01},
	langid = {english},
}

@article{rinonapoliStemCellsApplication2021,
	title = {Stem cells application in meniscal tears: a systematic review of pre-clinical and clinical evidence},
	volume = {25},
	issn = {1128-3602, 2284-0729},
	url = {https://doi.org/10.26355/eurrev_202112_27622},
	doi = {10.26355/eurrev_202112_27622},
	shorttitle = {Stem cells application in meniscal tears},
	abstract = {{OBJECTIVE}: Conservative and surgical treatments for meniscal lesions are various and this field of orthopedic surgery is in continuous development. Stem cells represent one of the current options to stimulate meniscal healing. The present systematic review aimed at summarizing the state of art in the application of stem cells for the treatment of meniscal damage both at pre-clinical and clinical level. {MATERIALS} {AND} {METHODS}: The {PRISMA} (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines were followed to perform this systematic review. A systematic search was performed using the {PubMed} ({MEDLINE}), {EMBASE} and Cochrane Library databases. All the studies dealing with the application of stem cells as a treatment for meniscal tears were pooled, data were extracted and analyzed. The studies were divided into two groups (pre-clinical and clinical), and then, discussed independently. {RESULTS}: A total of 18 studies were included. Thirteen were classified as “pre-clinical” and five as “clinical”. The most commonly used cells were mesenchymal stem cells ({MSC}), derived from bone marrow ({BMMSC}), synovial tissue ({SMSC}), or adipose tissue ({ADSC}). Follow-ups ranged from 2 to 16 weeks for the pre-clinical studies and from 3 to 24 months for the clinical studies. All studies documented good results in terms of laboratory markers/scores, clinical and radiologic evaluation. {CONCLUSIONS}: Based on the currently available data, it is not possible to establish the best cell source or delivery method for the treatment of meniscal injuries. Bone Marrow derived stem cells delivered through injection represent the most studied approach, with the most promising results. However, the full impact of these therapies through their different sub-type of stem cells and implantation techniques still needs to be critically analyzed through larger randomized controlled trials with longer follow-up.},
	pages = {7754--7764},
	number = {24},
	journaltitle = {European Review for Medical and Pharmacological Sciences},
	author = {Rinonapoli, G. and Gregori, P. and Di Matteo, B. and Impieri, L. and Ceccarini, P. and Manfreda, F. and Campofreda, G. and Caraffa, A.},
	urldate = {2022-03-17},
	date = {2021-12},
}

@article{abbadessaEngineeringAnisotropicMeniscus2020,
	title = {Engineering Anisotropic Meniscus: Zonal Functionality and Spatiotemporal Drug Delivery},
	issn = {1937-3368, 1937-3376},
	url = {https://www.liebertpub.com/doi/10.1089/ten.teb.2020.0096},
	doi = {10.1089/ten.teb.2020.0096},
	shorttitle = {Engineering Anisotropic Meniscus},
	journaltitle = {Tissue Engineering Part B: Reviews},
	shortjournal = {Tissue Engineering Part B: Reviews},
	author = {Abbadessa, Anna and Crecente-Campo, José and Alonso, María José},
	urldate = {2020-11-30},
	date = {2020-08-26},
	langid = {english},
}

@article{giuffridaConservativeVsSurgical2020,
	title = {Conservative vs. surgical approach for degenerative meniscal injuries: a systematic review of clinical evidence},
	volume = {24},
	issn = {1128-3602, 2284-0729},
	url = {https://doi.org/10.26355/eurrev_202003_20651},
	doi = {10.26355/eurrev_202003_20651},
	shorttitle = {Conservative vs. surgical approach for degenerative meniscal injuries},
	abstract = {{OBJECTIVE}: Analyzing the available evidence by comparing the role of arthroscopic surgery and conservative treatment in the management of degenerative meniscopathy. {MATERIALS} {AND} {METHODS}: A literature search was carried out on the {PubMed}, {EMBASE}, Scopus, and {PEDro} databases in May 2019 to identify all the randomized controlled trials ({RCTs}) comparing arthroscopic surgery to conservative management of painful but stable degenerated menisci. The quality of the {RCTs} was assessed using the Cochrane Risk of Bias Assessment. {RESULTS}: A total of 10 studies, including 1525 patients and dealing with conservative treatment vs. arthroscopic surgery were included in this review. In eight studies the effectiveness of exercise therapy was compared to surgery; in one study the effectiveness of intra-articular steroid injection was compared to surgery; in one study the effectiveness of placebo surgery was compared to partial meniscectomy. In all studies, no significant inter-group difference in terms of knee pain and knee function were observed at any follow-up evaluation. {CONCLUSIONS}: Degenerative meniscal tears, without symptoms of locking and catching, can be successfully managed by a proper regimen of physical therapy as a first line treatment. Surgical approach might be considered in case of poor response after conservative treatment.},
	pages = {2874--2885},
	number = {6},
	journaltitle = {European Review for Medical and Pharmacological Sciences},
	author = {Giuffrida, A. and Di Bari, A. and Falzone, E. and Iacono, F. and Kon, E. and Marcacci, M. and Gatti, R. and Di Matteo, B.},
	urldate = {2020-11-19},
	date = {2020-03},
}