TY - JOUR TI - Bioprinting Neural Systems to Model Central Nervous System Diseases AU - Qiu, Boning AU - Bessler, Nils AU - Figler, Kianti AU - Buchholz, Maj-Britt AU - Rios, Anne C. AU - Malda, Jos AU - Levato, Riccardo AU - Caiazzo, Massimiliano T2 - Advanced Functional Materials AB - To date, pharmaceutical progresses in central nervous system (CNS) diseases are clearly hampered by the lack of suitable disease models. Indeed, animal models do not faithfully represent human neurodegenerative processes and human in vitro 2D cell culture systems cannot recapitulate the in vivo complexity of neural systems. The search for valuable models of neurodegenerative diseases has recently been revived by the addition of 3D culture that allows to re-create the in vivo microenvironment including the interactions among different neural cell types and the surrounding extracellular matrix (ECM) components. In this review, the new challenges in the field of CNS diseases in vitro 3D modeling are discussed, focusing on the implementation of bioprinting approaches enabling positional control on the generation of the 3D microenvironments. The focus is specifically on the choice of the optimal materials to simulate the ECM brain compartment and the biofabrication technologies needed to shape the cellular components within a microenvironment that significantly represents brain biochemical and biophysical parameters. DA - 2020/// PY - 2020 DO - 10.1002/adfm.201910250 DP - Wiley Online Library VL - 30 IS - 44 SP - 1910250 LA - en SN - 1616-3028 UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201910250 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/34566552/ DB - PMC Y2 - 2021/01/08/18:10:53 KW - 3D culture KW - Parkinson's disease KW - biofabrication KW - disease modeling KW - hydrogels ER - TY - JOUR TI - From Shape to Function: The Next Step in Bioprinting AU - Levato, Riccardo AU - Jungst, Tomasz AU - Scheuring, Ruben G. AU - Blunk, Torsten AU - Groll, Juergen AU - Malda, Jos T2 - Advanced Materials AB - In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell-laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined. DA - 2020/// PY - 2020 DO - 10.1002/adma.201906423 DP - Wiley Online Library VL - 32 IS - 12 SP - 1906423 LA - en SN - 1521-4095 ST - From Shape to Function UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201906423 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32045053/ DB - PMC Y2 - 2021/01/08/18:08:28 KW - biofabrication KW - bioinks KW - biological function KW - regenerative medicine KW - tissue hierarchy ER - TY - JOUR TI - Biofunctionalization of 3D printed collagen with bevacizumab-loaded microparticles targeting pathological angiogenesis AU - Abbadessa, Anna AU - Nuñez Bernal, Paulina AU - Buttitta, Giorgio AU - Ronca, Alfredo AU - D'Amora, Ugo AU - Zihlmann, Carla AU - Stiefel, Niklaus AU - Ambrosio, Luigi AU - Malda, Jos AU - Levato, Riccardo AU - Crecente-Campo, José AU - Alonso, María José T2 - Journal of Controlled Release DA - 2023/08// PY - 2023 DO - 10.1016/j.jconrel.2023.07.017 DP - DOI.org (Crossref) VL - 360 SP - 747 EP - 758 J2 - Journal of Controlled Release LA - en SN - 01683659 UR - https://linkinghub.elsevier.com/retrieve/pii/S0168365923004443 AN - https://zenodo.org/record/8167346 DB - Zenodo Y2 - 2023/07/20/09:27:01 ER - TY - THES TI - Crafting Tissue Complexity: Pioneering Layerwise and Volumetric Biofabrication Strategies for Advanced In Vitro Models AU - Núñez Bernal, Paulina AB - The global rise in life expectancy is accompanied by an increasing prevalence of chronic diseases, posing economic burdens and impacting patients' well-being. This surge in disease incidence challenges the drug discovery pipeline, calling for the adaptation of its rules and regulations to reduce the ever-increasing failure rates of new drugs. The staggering growth of the pharmaceutical industry in the last decades, and the high expenditure required to bring new drugs to the market is set to become an unsustainable issue in the coming years. Recently, far more attention has been focused on the preclinical phase of the pipeline, where drug evaluation culminates in animal testing, the current gold standard to ensure drug safety and efficacy before human trials. A consensus on the need for more complex and predictive human disease models has emerged and become a priority for scientists and policy makers in the past decade. Biofabrication, an emerging technology-driven field, has gained prominence in biomedical research for developing advanced in vitro models. Biofabrication is “the automated generation of biologically functional products with structural organization from living cells, bioactive molecules, biomaterials, cell aggregates such as micro-tissues, or hybrid cell-material constructs, through Bioprinting or Bioassembly and subsequent tissue maturation processes”. The last decades have seen the rapid development new bioprinting modalities, opening the door to the development of architecturally complex in vitro platforms where multi-cellular and multi-material structures can be more easily created. Considering the need for more complex preclinical models to bridge the translational gap between 2D in vitro models, animal testing and clinical trials, the overarching aim of this thesis was “to develop new biofabrication approaches, encompassing 3D bioprinting technologies, powerful biological building blocks, and smart biomaterials, that facilitate the development of advanced human in vitro models with native tissue-like functionality”. This research has introduced significant advancements within the field of biofabrication for the generation of human in vitro models. Through a comprehensive exploration that tackled challenges related to fundamental bioprinting principles, the biological intricacies, and the biochemical and material property requirements of bioprinted structures to better recapitulate native tissues, the developments described here have expanded the toolkit of biofabrication approaches. By introducing novel technologies, like the pioneering, layerless volumetric bioprinting strategy, and synergizing new and existing printing approaches to harness their unique advantages, this thesis showcases a variety of functional bioprinted tissue models that enable the study of biological processes in vitro. The thorough exploration of volumetric bioprinting, distinguished by its scalability, unparalleled design freedom, compatibility with advanced biological tools, and a growing library of smart materials, charts new paths toward the creation of clinically-relevant testing platforms. The bioprinted, tissue-specific in vitro models presented here offer enhanced physiological accuracy and predictability and hold the potential to incorporate patient-specific elements for personalized medicine. The toolkit developed in here represents a significant stride in bridging the translational gap of tissue-engineered in vitro models, particularly in the context of preclinical testing. All in all, the evolving landscape of biofabricated in vitro models holds promise for innovative approaches in the future. DA - 2024/01/10/ PY - 2024 DO - 10.33540/2116 DP - DOI.org (Crossref) LA - en M3 - dr. PB - Utrecht University SN - 978-90-393-7622-5 ST - Crafting Tissue Complexity UR - https://dspace.library.uu.nl/handle/1874/433677 AN - https://dspace.library.uu.nl/bitstream/handle/1874/433677/pnunezbernalcrafting%20tissue%20complexity2024sharing%20-%20659c1caac8491.pdf?sequence=1&isAllowed=y DB - UU repository Y2 - 2024/12/04/14:58:12 ER - TY - JOUR TI - Three‐dimensional bone morphology is a risk factor for medial postmeniscectomy syndrome: A retrospective cohort study AU - Grammens, Jonas AU - Van Haver, Annemieke AU - Danckaers, Femke AU - Vuylsteke, Kristien AU - Sijbers, Jan AU - Mahluf, Lotem AU - Angele, Peter AU - Kon, Elizaveta AU - Verdonk, Peter AU - MEFISTO WP1 Group T2 - Journal of Experimental Orthopaedics AB - Abstract Purpose The study aims to identify differences in tibiofemoral joint morphology between responders (R group, no pain) to arthroscopic partial medial meniscectomy (APMM) versus medial postmeniscectomy syndrome patients (MPMS group, recurrent pain at 2 years postmeniscectomy) in a clinically neutrally aligned patient population. The second aim was to build a morphology‐based predictive algorithm for response to treatment (RTT) in APMM. Methods Two patient groups were identified from a large multicentre database of meniscectomy patients at 2 years of follow‐up: the R group included 120 patients with a KOOS pain score > 75, and the MPMS group included 120 patients with a KOOS pain score ≤ 75. Statistical shape models (SSMs) of distal femur, proximal tibia and tibiofemoral joint were used to compare knee morphology. Finally, a predictive model was developed to predict RTT, with the SSM‐derived morphologic variables as predictors. Results No differences were found between the R and MPMS groups for patient age, sex, height, weight or cartilage status. Knees in the MPMS group were significantly smaller, had a wider femoral notch and a smaller medial femoral condyle. A morphology‐based predictive model was able to predict MPMS at 2 years follow‐up with a sensitivity of 74.9% (95% confidence interval [CI]: 74.4%–75.4%) and a specificity of 81.0% (95% CI: 80.6%–81.5%). Conclusion A smaller tibiofemoral joint, a wider intercondylar notch and smaller medial femoral condyle were observed shape variations related to medial postmeniscectomy syndrome. These promising results are a first step towards a knee morphology‐based clinical decision support tool for meniscus treatment. Study Design Case–control study. Level of Evidence Level IIIb. DA - 2024/07// PY - 2024 DO - 10.1002/jeo2.12090 DP - DOI.org (Crossref) VL - 11 IS - 3 SP - e12090 J2 - J. exp. orthop. LA - en SN - 2197-1153, 2197-1153 ST - Three‐dimensional bone morphology is a risk factor for medial postmeniscectomy syndrome UR - https://esskajournals.onlinelibrary.wiley.com/doi/10.1002/jeo2.12090 AN - https://pmc.ncbi.nlm.nih.gov/articles/PMC11260280/ DB - PMC Y2 - 2024/12/04/14:33:47 ER - TY - JOUR TI - Orthobiologic injections for treating degenerative meniscus lesions: a matter of facts? Ten years of clinical experience in a systematic review AU - Conte, Pietro AU - Anzillotti, Giuseppe AU - Di Matteo, Berardo AU - Gallese, Alessandro AU - Vitale, Umberto AU - Marcacci, Maurilio AU - Kon, Elizaveta T2 - Journal of Cartilage & Joint Preservation DA - 2023/01// PY - 2023 DO - 10.1016/j.jcjp.2023.100104 DP - DOI.org (Crossref) SP - 100104 J2 - Journal of Cartilage & Joint Preservation LA - en SN - 26672545 ST - Orthobiologic injections for treating degenerative meniscus lesions UR - https://linkinghub.elsevier.com/retrieve/pii/S2667254523000069 AN - https://zenodo.org/records/11634197 DB - Zenodo Y2 - 2023/04/03/09:15:37 ER - TY - JOUR TI - Volumetric Bioprinting of Complex Living-Tissue Constructs within Seconds AU - Bernal, Paulina Nuñez AU - Delrot, Paul AU - Loterie, Damien AU - Li, Yang AU - Malda, Jos AU - Moser, Christophe AU - Levato, Riccardo T2 - Advanced Materials AB - Biofabrication technologies, including stereolithography and extrusion-based printing, are revolutionizing the creation of complex engineered tissues. The current paradigm in bioprinting relies on the additive layer-by-layer deposition and assembly of repetitive building blocks, typically cell-laden hydrogel fibers or voxels, single cells, or cellular aggregates. The scalability of these additive manufacturing technologies is limited by their printing velocity, as lengthy biofabrication processes impair cell functionality. Overcoming such limitations, the volumetric bioprinting of clinically relevant sized, anatomically shaped constructs, in a time frame ranging from seconds to tens of seconds is described. An optical-tomography-inspired printing approach, based on visible light projection, is developed to generate cell-laden tissue constructs with high viability (>85%) from gelatin-based photoresponsive hydrogels. Free-form architectures, difficult to reproduce with conventional printing, are obtained, including anatomically correct trabecular bone models with embedded angiogenic sprouts and meniscal grafts. The latter undergoes maturation in vitro as the bioprinted chondroprogenitor cells synthesize neo-fibrocartilage matrix. Moreover, free-floating structures are generated, as demonstrated by printing functional hydrogel-based ball-and-cage fluidic valves. Volumetric bioprinting permits the creation of geometrically complex, centimeter-scale constructs at an unprecedented printing velocity, opening new avenues for upscaling the production of hydrogel-based constructs and for their application in tissue engineering, regenerative medicine, and soft robotics. DA - 2019/// PY - 2019 DO - 10.1002/adma.201904209 DP - Wiley Online Library VL - 31 IS - 42 SP - 1904209 LA - en SN - 1521-4095 UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201904209 AN - https://infoscience.epfl.ch/record/270068 DB - Infoscience Y2 - 2019/11/20/09:23:08 KW - biofabrication KW - bioinks KW - bioresins KW - cell encapsulation KW - photopolymers KW - tomographic laser prototyping ER - TY - JOUR TI - Small medial femoral condyle morphotype is associated with medial compartment degeneration and distinct morphological characteristics: a comparative pilot study AU - Grammens, Jonas AU - Van Haver, Annemieke AU - Danckaers, Femke AU - Booth, Brian AU - Sijbers, Jan AU - Verdonk, Peter T2 - Knee Surgery, Sports Traumatology, Arthroscopy AB - Early-onset degeneration of the knee is linked to genetics, overload, injury, and potentially, knee morphology. The purpose of this study is to explore the characteristics of the small medial femoral condyle, as a distinct knee morphotype, by means of a landmark-based three-dimensional (3D) analysis and statistical parametric mapping. DA - 2020/08/14/ PY - 2020 DO - 10.1007/s00167-020-06218-8 DP - Springer Link J2 - Knee Surg Sports Traumatol Arthrosc LA - en SN - 1433-7347 ST - Small medial femoral condyle morphotype is associated with medial compartment degeneration and distinct morphological characteristics UR - https://doi.org/10.1007/s00167-020-06218-8 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32797248/ DB - PMC Y2 - 2020/09/02/17:43:38 ER - TY - JOUR TI - One‐Step Photoactivation of a Dual‐Functionalized Bioink as Cell Carrier and Cartilage‐Binding Glue for Chondral Regeneration AU - Lim, Khoon S. AU - Abinzano, Florencia AU - Bernal, Paulina Nuñez AU - Albillos Sanchez, Ane AU - Atienza‐Roca, Pau AU - Otto, Iris A. AU - Peiffer, Quentin C. AU - Matsusaki, Michiya AU - Woodfield, Tim B. F. AU - Malda, Jos AU - Levato, Riccardo T2 - Advanced Healthcare Materials DA - 2020/08// PY - 2020 DO - 10.1002/adhm.201901792 DP - DOI.org (Crossref) VL - 9 IS - 15 SP - 1901792 J2 - Adv. Healthcare Mater. LA - en SN - 2192-2640, 2192-2659 UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.201901792 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32324342/ DB - PMC Y2 - 2020/12/01/13:33:56 ER - TY - JOUR TI - Physioxia Expanded Bone Marrow Derived Mesenchymal Stem Cells Have Improved Cartilage Repair in an Early Osteoarthritic Focal Defect Model AU - Pattappa, Girish AU - Krueckel, Jonas AU - Schewior, Ruth AU - Franke, Dustin AU - Mench, Alexander AU - Koch, Matthias AU - Weber, Johannes AU - Lang, Siegmund AU - Pfeifer, Christian G. AU - Johnstone, Brian AU - Docheva, Denitsa AU - Alt, Volker AU - Angele, Peter AU - Zellner, Johannes T2 - Biology AB - Focal early osteoarthritis (OA) or degenerative lesions account for 60% of treated cartilage defects each year. The current cell-based regenerative treatments have an increased failure rate for treating degenerative lesions compared to traumatic defects. Mesenchymal stem cells (MSCs) are an alternative cell source for treating early OA defects, due to their greater chondrogenic potential, compared to early OA chondrocytes. Low oxygen tension or physioxia has been shown to enhance MSC chondrogenic matrix content and could improve functional outcomes of regenerative therapies. The present investigation sought to develop a focal early OA animal model to evaluate cartilage regeneration and hypothesized that physioxic MSCs improve in vivo cartilage repair in both, post-trauma and focal early OA defects. Using a rabbit model, a focal defect was created, that developed signs of focal early OA after six weeks. MSCs cultured under physioxia had significantly enhanced in vitro MSC chondrogenic GAG content under hyperoxia with or without the presence of interleukin-1β (IL-1β). In both post-traumatic and focal early OA defect models, physioxic MSC treatment demonstrated a significant improvement in cartilage repair score, compared to hyperoxic MSCs and respective control defects. Future investigations will seek to understand whether these results are replicated in large animal models and the underlying mechanisms involved in in vivo cartilage regeneration. DA - 2020/08// PY - 2020 DO - 10.3390/biology9080230 DP - www.mdpi.com VL - 9 IS - 8 SP - 230 LA - en PB - Multidisciplinary Digital Publishing Institute UR - https://www.mdpi.com/2079-7737/9/8/230 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32824442/ DB - PMC Y2 - 2020/09/02/17:44:51 KW - cartilage KW - chondrogenesis KW - disease& KW - early osteoarthritis KW - hypoxia KW - mesenchymal stem cells KW - modeling ER - TY - JOUR TI - Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis AU - Ummarino, Aldo AU - Gambaro, Francesco Manlio AU - Kon, Elizaveta AU - Torres Andón, Fernando T2 - Nanomaterials AB - Osteoarthritis (OA) is the most common joint pathology causing severe pain and disability. Macrophages play a central role in the pathogenesis of OA. In the joint microenvironment, macrophages with an M1-like pro-inflammatory phenotype induce chronic inflammation and joint destruction, and they have been correlated with the development and progression of the disease, while the M2-like anti-inflammatory macrophages support the recovery of the disease, promoting tissue repair and the resolution of inflammation. Nowadays, the treatment of OA in the clinic relies on systemic and/or intra-articular administration of anti-inflammatory and pain relief drugs, as well as surgical interventions for the severe cases (i.e., meniscectomy). The disadvantages of the pharmacological therapy are related to the chronic nature of the disease, requiring prolonged treatments, and to the particular location of the pathology in joint tissues, which are separated anatomical compartments with difficult access for the drugs. To overcome these challenges, nanotechnological approaches have been investigated to improve the delivery of drugs toward macrophages into the diseased joint. This strategy may offer advantages by reducing off-target toxicities and improving long-term therapeutic efficacy. In this review, we describe the nanomaterial-based approaches designed so far to directly or indirectly manipulate macrophages for the treatment of osteoarthritis. DA - 2020/08// PY - 2020 DO - 10.3390/nano10081562 DP - www.mdpi.com VL - 10 IS - 8 SP - 1562 LA - en PB - Multidisciplinary Digital Publishing Institute UR - https://www.mdpi.com/2079-4991/10/8/1562 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32784839/ DB - PMC Y2 - 2020/09/02/17:44:21 KW - anti-inflammatory KW - drug delivery KW - immune system KW - innate immunity KW - macrophage KW - nanomaterial KW - nanoparticle KW - osteoarthritis ER - TY - JOUR TI - Cell-based treatment options facilitate regeneration of cartilage, ligaments and meniscus in demanding conditions of the knee by a whole joint approach AU - Angele, Peter AU - Docheva, Denitsa AU - Pattappa, Girish AU - Zellner, Johannes T2 - Knee Surgery, Sports Traumatology, Arthroscopy AB - Abstract Purpose This article provides an update on the current therapeutic options for cell-based regenerative treatment of the knee with a critical review of the present literature including a future perspective on the use of regenerative cell-based approaches. Special emphasis has been given on the requirement of a whole joint approach with treatment of comorbidities with aim of knee cartilage restoration, particularly in demanding conditions like early osteoarthritis. Methods This narrative review evaluates recent clinical data and published research articles on cell-based regenerative treatment options for cartilage and other structures around the knee Results Cell-based regenerative therapies for cartilage repair have become standard practice for the treatment of focal, traumatic chondral defects of the knee. Specifically, matrix-assisted autologous chondrocyte transplantation (MACT) shows satisfactory long-term results regarding radiological, histological and clinical outcome for treatment of large cartilage defects. Data show that regenerative treatment of the knee requires a whole joint approach by addressing all comorbidities including axis deviation, instability or meniscus pathologies. Further development of novel biomaterials and the discovery of alternative cell sources may facilitate the process of cell-based regenerative therapies for all knee structures becoming the gold standard in the future. Conclusion Overall, cell-based regenerative cartilage therapy of the knee has shown tremendous development over the last years and has become the standard of care for large and isolated chondral defects. It has shown success in the treatment of traumatic, osteochondral defects but also for degenerative cartilage lesions in the demanding condition of early OA. Future developments and alternative cell sources may help to facilitate cell-based regenerative treatment for all different structures around the knee by a whole joint approach. Level of evidence IV. DA - 2021/03/05/ PY - 2021 DO - 10.1007/s00167-021-06497-9 DP - DOI.org (Crossref) J2 - Knee Surg Sports Traumatol Arthrosc LA - en SN - 0942-2056, 1433-7347 UR - https://link.springer.com/10.1007/s00167-021-06497-9 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/33666685/ DB - PMC Y2 - 2021/06/04/07:10:52 ER - TY - JOUR TI - Hydrogel-Based Bioinks for Cell Electrowriting of Well-Organized Living Structures with Micrometer-Scale Resolution AU - Castilho, Miguel AU - Levato, Riccardo AU - Bernal, Paulina Nunez AU - de Ruijter, Mylène AU - Sheng, Christina Y. AU - van Duijn, Joost AU - Piluso, Susanna AU - Ito, Keita AU - Malda, Jos T2 - Biomacromolecules DA - 2021/01/07/ PY - 2021 DO - 10.1021/acs.biomac.0c01577 DP - DOI.org (Crossref) SP - acs.biomac.0c01577 J2 - Biomacromolecules LA - en SN - 1525-7797, 1526-4602 UR - https://pubs.acs.org/doi/10.1021/acs.biomac.0c01577 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/33412840/ DB - PMC Y2 - 2021/01/15/13:28:06 ER - TY - JOUR TI - Drug Delivery Systems for the Treatment of Knee Osteoarthritis: A Systematic Review of In Vivo Studies AU - Gambaro, Francesco Manlio AU - Ummarino, Aldo AU - Torres Andón, Fernando AU - Ronzoni, Flavio AU - Di Matteo, Berardo AU - Kon, Elizaveta T2 - International Journal of Molecular Sciences AB - Many efforts have been made in the field of nanotechnology to improve the local and sustained release of drugs, which may be helpful to overcome the present limitations in the treatment of knee OA. Nano-/microparticles and/or hydrogels can be now engineered to improve the administration and intra-articular delivery of specific drugs, targeting molecular pathways and pathogenic mechanisms involved in OA progression and remission. In order to summarize the current state of this field, a systematic review of the literature was performed and 45 relevant studies were identified involving both animal models and humans. We found that polymeric nanoparticles loaded with anti-inflammatory drugs (i.e., dexamethasone or celecoxib) are the most frequently investigated drug delivery systems, followed by microparticles and hydrogels. In particular, the nanosystem most frequently used in preclinical research consists of PLGA-nanoparticles loaded with corticosteroids and non-steroidal anti-inflammatory drugs. Overall, improvement in histological features, reduction in joint inflammation, and improvement in clinical scores in patients were observed. The last advances in the field of nanotechnology could offer new opportunities to treat patients affected by knee OA, including those with previous meniscectomy. New smart drug delivery approaches, based on nanoparticles, microparticles, and hydrogels, may enhance the therapeutic potential of intra-articular agents by increasing the permanence of selected drugs inside the joint and better targeting specific receptors and tissues. DA - 2021/08/24/ PY - 2021 DO - 10.3390/ijms22179137 DP - DOI.org (Crossref) VL - 22 IS - 17 SP - 9137 J2 - IJMS LA - en SN - 1422-0067 ST - Drug Delivery Systems for the Treatment of Knee Osteoarthritis UR - https://www.mdpi.com/1422-0067/22/17/9137 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/34502046/ DB - PMC Y2 - 2022/03/17/15:22:10 ER - TY - JOUR TI - Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin: A Novel Approach in Meniscus Tissue Engineering AU - Korpershoek, Jasmijn V. AU - Rikkers, Margot AU - de Windt, Tommy S. AU - Tryfonidou, Marianna A. AU - Saris, Daniel B. F. AU - Vonk, Lucienne A. T2 - International Journal of Molecular Sciences AB - Meniscus injuries can be highly debilitating and lead to knee osteoarthritis. Progenitor cells from the meniscus could be a superior cell type for meniscus repair and tissue-engineering. The purpose of this study is to characterize meniscus progenitor cells isolated by differential adhesion to fibronectin (FN-prog). Human osteoarthritic menisci were digested, and FN-prog were selected by differential adhesion to fibronectin. Multilineage differentiation, population doubling time, colony formation, and MSC surface markers were assessed in the FN-prog and the total meniscus population (Men). Colony formation was compared between outer and inner zone meniscus digest. Chondrogenic pellet cultures were performed for redifferentiation. FN-prog demonstrated multipotency. The outer zone FN-prog formed more colonies than the inner zone FN-prog. FN-prog displayed more colony formation and a higher proliferation rate than Men. FN-prog redifferentiated in pellet culture and mostly adhered to the MSC surface marker profile, except for HLA-DR receptor expression. This is the first study that demonstrates differential adhesion to fibronectin for the isolation of a progenitor-like population from the meniscus. The high proliferation rates and ability to form meniscus extracellular matrix upon redifferentiation, together with the broad availability of osteoarthritis meniscus tissue, make FN-prog a promising cell type for clinical translation in meniscus tissue-engineering. DA - 2021/08/10/ PY - 2021 DO - 10.3390/ijms22168614 DP - DOI.org (Crossref) VL - 22 IS - 16 SP - 8614 J2 - IJMS LA - en SN - 1422-0067 ST - Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin UR - https://www.mdpi.com/1422-0067/22/16/8614 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/34445320/ DB - PMC Y2 - 2021/10/19/16:28:26 ER - TY - JOUR TI - Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells AU - Korpershoek, Jasmijn V. AU - Ruijter, Mylène de AU - Terhaard, Bastiaan F. AU - Hagmeijer, Michella H. AU - Saris, Daniël B.F. AU - Castilho, Miguel AU - Malda, Jos AU - Vonk, Lucienne A. T2 - International Journal of Molecular Sciences AB - Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young’s modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment. DA - 2021/10/18/ PY - 2021 DO - 10.3390/ijms222011200 DP - DOI.org (Crossref) VL - 22 IS - 20 SP - 11200 J2 - IJMS LA - en SN - 1422-0067 UR - https://www.mdpi.com/1422-0067/22/20/11200 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/34681860/ DB - PMC Y2 - 2022/05/30/14:14:35 ER - TY - JOUR TI - Progenitor Cells in Healthy and Osteoarthritic Human Cartilage Have Extensive Culture Expansion Capacity while Retaining Chondrogenic Properties AU - Rikkers, M. AU - Korpershoek, J.V. AU - Levato, R. AU - Malda, J. AU - Vonk, L.A. T2 - CARTILAGE AB - Objective Articular cartilage-derived progenitor cells (ACPCs) are a potential new cell source for cartilage repair. This study aims to characterize endogenous ACPCs from healthy and osteoarthritic (OA) cartilage, evaluate their potential for cartilage regeneration, and compare this to cartilage formation by chondrocytes. Design ACPCs were isolated from full-thickness healthy and OA human cartilage and separated from the total cell population by clonal growth after differential adhesion to fibronectin. ACPCs were characterized by growth kinetics, multilineage differentiation, and surface marker expression. Chondrogenic redifferentiation of ACPCs was compared with chondrocytes in pellet cultures. Pellets were assessed for cartilage-like matrix production by (immuno)histochemistry, quantitative analyses for glycosaminoglycans and DNA content, and expression of chondrogenic and hypertrophic genes. Results Healthy and OA ACPCs were successfully differentiated toward the adipogenic and chondrogenic lineage, but failed to produce calcified matrix when exposed to osteogenic induction media. Both ACPC populations met the criteria for cell surface marker expression of mesenchymal stromal cells (MSCs). Healthy ACPCs cultured in pellets deposited extracellular matrix containing proteoglycans and type II collagen, devoid of type I collagen. Gene expression of hypertrophic marker type X collagen was lower in healthy ACPC pellets compared with OA pellets. Conclusions This study provides further insight into the ACPC population in healthy and OA human articular cartilage. ACPCs show similarities to MSCs, yet do not produce calcified matrix under well-established osteogenic culture conditions. Due to extensive proliferative potential and chondrogenic capacity, ACPCs show potential for cartilage regeneration and possibly for clinical application, as a promising alternative to MSCs or chondrocytes. DA - 2021/12// PY - 2021 DO - 10.1177/19476035211059600 DP - DOI.org (Crossref) VL - 13 IS - 2_suppl SP - 129S EP - 142S J2 - CARTILAGE LA - en SN - 1947-6035, 1947-6043 UR - http://journals.sagepub.com/doi/10.1177/19476035211059600 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/34802263/ DB - PMC Y2 - 2022/03/17/15:19:42 ER - TY - JOUR TI - Biosynthetic scaffolds for partial meniscal loss: A systematic review from animal models to clinical practice AU - Veronesi, F. AU - Di Matteo, B. AU - Vitale, N.D. AU - Filardo, G. AU - Visani, A. AU - Kon, E. AU - Fini, M. T2 - Bioactive Materials DA - 2021/11// PY - 2021 DO - 10.1016/j.bioactmat.2021.03.033 DP - DOI.org (Crossref) VL - 6 IS - 11 SP - 3782 EP - 3800 J2 - Bioactive Materials LA - en SN - 2452199X ST - Biosynthetic scaffolds for partial meniscal loss UR - https://linkinghub.elsevier.com/retrieve/pii/S2452199X21001444 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/33898878/ DB - PMC Y2 - 2021/06/04/06:53:20 ER - TY - JOUR TI - Single Cell Bioprinting with Ultrashort Laser Pulses AU - Zhang, Jun AU - Byers, Patrick AU - Erben, Amelie AU - Frank, Christine AU - Schulte‐Spechtel, Levin AU - Heymann, Michael AU - Docheva, Denitsa AU - Huber, Heinz P. AU - Sudhop, Stefanie AU - Clausen‐Schaumann, Hauke T2 - Advanced Functional Materials DA - 2021/05// PY - 2021 DO - 10.1002/adfm.202100066 DP - DOI.org (Crossref) VL - 31 IS - 19 SP - 2100066 J2 - Adv. Funct. Mater. LA - en SN - 1616-301X, 1616-3028 UR - https://onlinelibrary.wiley.com/doi/10.1002/adfm.202100066 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538086/ DB - PMC Y2 - 2022/03/17/15:36:44 ER - TY - JOUR TI - Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations AU - Zhang, Jun AU - Geiger, Yasemin AU - Sotier, Florian AU - Djordjevic, Sasa AU - Docheva, Denitsa AU - Sudhop, Stefanie AU - Clausen-Schaumann, Hauke AU - Huber, Heinz P. T2 - Micromachines AB - Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rate after transfer above 95% through the entire pulse duration range. More cost efficient and compact laser sources with pulse durations in the picosecond range could be used for film-free bioprinting and single-cell transfer. DA - 2021/09/29/ PY - 2021 DO - 10.3390/mi12101172 DP - DOI.org (Crossref) VL - 12 IS - 10 SP - 1172 J2 - Micromachines LA - en SN - 2072-666X UR - https://www.mdpi.com/2072-666X/12/10/1172 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/34683222/ DB - PMC Y2 - 2022/03/17/15:46:37 ER - TY - JOUR TI - Fibronectin Adherent Cell Populations Derived From Avascular and Vascular Regions of the Meniscus Have Enhanced Clonogenicity and Differentiation Potential Under Physioxia AU - Pattappa, Girish AU - Reischl, Franziska AU - Jahns, Judith AU - Schewior, Ruth AU - Lang, Siegmund AU - Zellner, Johannes AU - Johnstone, Brian AU - Docheva, Denitsa AU - Angele, Peter T2 - Frontiers in Bioengineering and Biotechnology AB - The meniscus is composed of an avascular inner region and vascular outer region. The vascular region has been shown to contain a progenitor population with multilineage differentiation capacity. Strategies facilitating the isolation and propagation of these progenitors can be used to develop cell-based meniscal therapies. Differential adhesion to fibronectin has been used to isolate progenitor populations from cartilage, while low oxygen or physioxia (2% oxygen) enhances the meniscal phenotype. This study aimed to isolate progenitor populations from the avascular and vascular meniscus using differential fibronectin adherence and examine their clonogenicity and differentiation potential under hyperoxia (20% oxygen) and physioxia (2% oxygen). Human vascular and avascular meniscus cells were seeded onto fibronectin-coated dishes for a short period and monitored for colony formation under either hyperoxia or physioxia. Non-fibronectin adherent meniscus cells were also expanded under both oxygen tension. Individual fibronectin adherent colonies were isolated and further expanded, until approximately ten population doublings (passage 3), whereby they underwent chondrogenic, osteogenic, and adipogenic differentiation. Physioxia enhances clonogenicity of vascular and avascular meniscus cells on plastic or fibronectin-coated plates. Combined differential fibronectin adhesion and physioxia isolated a progenitor population from both meniscus regions with trilineage differentiation potential compared to equivalent hyperoxia progenitors. Physioxia isolated progenitors had a significantly enhanced meniscus matrix content without the presence of collagen X. These results demonstrate that combined physioxia and fibronectin adherence can isolate and propagate a meniscus progenitor population that can potentially be used to treat meniscal tears or defects. DA - 2022/01/28/ PY - 2022 DO - 10.3389/fbioe.2021.789621 DP - DOI.org (Crossref) VL - 9 SP - 789621 J2 - Front. Bioeng. Biotechnol. SN - 2296-4185 UR - https://www.frontiersin.org/articles/10.3389/fbioe.2021.789621/full AN - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/35155405/ DB - PMC Y2 - 2022/02/04/12:06:07 ER - TY - JOUR TI - The clinical potential of articular cartilage-derived progenitor cells: a systematic review AU - Rikkers, Margot AU - Korpershoek, Jasmijn V. AU - Levato, Riccardo AU - Malda, Jos AU - Vonk, Lucienne A. T2 - npj Regenerative Medicine AB - Abstract Over the past two decades, evidence has emerged for the existence of a distinct population of endogenous progenitor cells in adult articular cartilage, predominantly referred to as articular cartilage-derived progenitor cells (ACPCs). This progenitor population can be isolated from articular cartilage of a broad range of species, including human, equine, and bovine cartilage. In vitro, ACPCs possess mesenchymal stromal cell (MSC)-like characteristics, such as colony forming potential, extensive proliferation, and multilineage potential. Contrary to bone marrow-derived MSCs, ACPCs exhibit no signs of hypertrophic differentiation and therefore hold potential for cartilage repair. As no unique cell marker or marker set has been established to specifically identify ACPCs, isolation and characterization protocols vary greatly. This systematic review summarizes the state-of-the-art research on this promising cell type for use in cartilage repair therapies. It provides an overview of the available literature on endogenous progenitor cells in adult articular cartilage and specifically compares identification of these cell populations in healthy and osteoarthritic (OA) cartilage, isolation procedures, in vitro characterization, and advantages over other cell types used for cartilage repair. The methods for the systematic review were prospectively registered in PROSPERO (CRD42020184775). DA - 2022/01/10/ PY - 2022 DO - 10.1038/s41536-021-00203-6 DP - DOI.org (Crossref) VL - 7 IS - 1 SP - 2 J2 - npj Regen Med LA - en SN - 2057-3995 ST - The clinical potential of articular cartilage-derived progenitor cells UR - https://www.nature.com/articles/s41536-021-00203-6 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748760/ DB - PMC Y2 - 2023/10/20/09:40:18 ER - TY - JOUR TI - An in vitro model for osteoarthritis using long‐cultured inflammatory human macrophages repeatedly stimulated with TLR agonists AU - Ummarino, Aldo AU - Pensado‐López, Alba AU - Migliore, Roberta AU - Alcaide‐Ruggiero, Lourdes AU - Calà, Nicholas AU - Caputo, Michele AU - Gambaro, Francesco M. AU - Anfray, Clément AU - Ronzoni, Flavio L. AU - Kon, Elizaveta AU - Allavena, Paola AU - Torres Andón, Fernando T2 - European Journal of Immunology AB - Abstract Osteoarthritis (OA) is characterized by an abundance of inflammatory M1‐like macrophages damaging local tissues. The search for new potential drugs for OA suffers from the lack of appropriate methods of long‐lasting inflammation. Here we developed and characterized an in vitro protocol of long‐lasting culture of primary human monocyte‐derived macrophages differentiated with a combination of M‐CSF+GM‐CSF that optimally supported long‐cultured macrophages (LC‐Mϕs) for up to 15 days, unlike their single use. Macrophages repeatedly stimulated for 15 days with the TLR2 ligand Pam3CSK4 (LCS‐Mϕs), showed sustained levels over time of IL‐6, CCL2, and CXCL8, inflammatory mediators that were also detected in the synovial fluids of OA patients. Furthermore, macrophages isolated from the synovia of two OA patients showed an expression profile of inflammation‐related genes similar to that of LCS‐Mϕs, validating our protocol as a model of chronically activated inflammatory macrophages. Next, to confirm that these LCS‐Mϕs could be modulated by anti‐inflammatory compounds, we employed dexamethasone and/or celecoxib, two drugs widely used in OA treatment, that significantly inhibited the production of inflammatory mediators. This easy‐to‐use in vitro protocol of long‐lasting inflammation with primary human macrophages could be useful for the screening of new compounds to improve the therapy of inflammatory disorders. DA - 2023/10/06/ PY - 2023 DO - 10.1002/eji.202350507 DP - DOI.org (Crossref) SP - 2350507 J2 - Eur J Immunol LA - en SN - 0014-2980, 1521-4141 UR - https://onlinelibrary.wiley.com/doi/10.1002/eji.202350507 AN - https://zenodo.org/records/11395139 DB - Zenodo Y2 - 2023/10/10/09:27:40 ER - TY - JOUR TI - Bioink with cartilage-derived extracellular matrix microfibers enables spatial control of vascular capillary formation in bioprinted constructs AU - Terpstra, Margo L AU - Li, Jinyu AU - Mensinga, Anneloes AU - de Ruijter, Mylène AU - van Rijen, Mattie H P AU - Androulidakis, Charalampos AU - Galiotis, Costas AU - Papantoniou, Ioannis AU - Matsusaki, Michiya AU - Malda, Jos AU - Levato, Riccardo T2 - Biofabrication AB - Abstract Microvasculature is essential for the exchange of gas and nutrient for most tissues in our body. Some tissue structures such as the meniscus presents spatially confined blood vessels adjacent to non-vascularized regions. In biofabrication, mimicking the spatial distribution of such vascular components is paramount, as capillary ingrowth into non-vascularized tissues can lead to tissue matrix alterations and subsequent pathology. Multi-material three-dimensional (3D) bioprinting strategies have the potential to resolve anisotropic tissue features, although building complex constructs comprising stable vascularized and non-vascularized regions remains a major challenge to date. In this study, we developed endothelial cell-laden pro- and anti-angiogenic bioinks, supplemented with bioactive matrix-derived microfibers (MFs) that were created from type I collagen sponges (col-1) and cartilage decellularized extracellular matrix (CdECM), respectively. Human umbilical vein endothelial cell (HUVEC)-driven capillary networks started to form 2 d after bioprinting. Supplementing cartilage-derived MFs to endothelial-cell laden bioinks reduced the total length of neo-microvessels by 29%, and the number of microvessel junctions by 37% after 14 d, compared to bioinks with pro-angiogenic col-1 MFs. As a proof of concept, the bioinks were bioprinted into an anatomical meniscus shape with a biomimetic vascularized outer and non-vascularized inner region, using a gellan gum microgel suspension bath. These 3D meniscus-like constructs were cultured up to 14 d, with in the outer zone the HUVEC-, mural cell-, and col-1 MF-laden pro-angiogenic bioink, and in the inner zone a meniscus progenitor cell (MPC)- and CdECM MF-laden anti-angiogenic bioink, revealing successful spatial confinement of the nascent vascular network only in the outer zone. Further, to co-facilitate both microvessel formation and MPC-derived matrix formation, we formulated cell culture medium conditions with a temporal switch. Overall, this study provides a new strategy that could be applied to develop zonal biomimetic meniscal constructs. Moreover, the use of ECM-derived MFs to promote or inhibit capillary networks opens new possibilities for the biofabrication of tissues with anisotropic microvascular distribution. These have potential for many applications including in vitro models of vascular-to-avascular tissue interfaces, cancer progression, and for testing anti-angiogenic therapies. DA - 2022/07/01/ PY - 2022 DO - 10.1088/1758-5090/ac6282 DP - DOI.org (Crossref) VL - 14 IS - 3 SP - 034104 J2 - Biofabrication SN - 1758-5082, 1758-5090 UR - https://iopscience.iop.org/article/10.1088/1758-5090/ac6282 AN - https://zenodo.org/records/6418450 DB - Zenodo Y2 - 2022/05/30/13:59:57 ER - TY - JOUR TI - Development of a highly concentrated collagen ink for the creation of a 3D printed meniscus AU - Ronca, Alfredo AU - D'Amora, Ugo AU - Capuana, Elisa AU - Zihlmann, Carla AU - Stiefel, Niklaus AU - Pattappa, Girish AU - Schewior, Ruth AU - Docheva, Denitsa AU - Angele, Peter AU - Ambrosio, Luigi T2 - Heliyon DA - 2023/12// PY - 2023 DO - 10.1016/j.heliyon.2023.e23107 DP - DOI.org (Crossref) VL - 9 IS - 12 SP - e23107 J2 - Heliyon LA - en SN - 24058440 UR - https://linkinghub.elsevier.com/retrieve/pii/S240584402310315X AN - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746456/ DB - PMC Y2 - 2024/01/18/14:58:01 ER - TY - JOUR TI - Automated Landmark Annotation for Morphometric Analysis of Distal Femur and Proximal Tibia AU - Grammens, Jonas AU - Van Haver, Annemieke AU - Lumban-Gaol, Imelda AU - Danckaers, Femke AU - Verdonk, Peter AU - Sijbers, Jan T2 - Journal of Imaging AB - Manual anatomical landmarking for morphometric knee bone characterization in orthopedics is highly time-consuming and shows high operator variability. Therefore, automation could be a substantial improvement for diagnostics and personalized treatments relying on landmark-based methods. Applications include implant sizing and planning, meniscal allograft sizing, and morphological risk factor assessment. For twenty MRI-based 3D bone and cartilage models, anatomical landmarks were manually applied by three experts, and morphometric measurements for 3D characterization of the distal femur and proximal tibia were calculated from all observations. One expert performed the landmark annotations three times. Intra- and inter-observer variations were assessed for landmark position and measurements. The mean of the three expert annotations served as the ground truth. Next, automated landmark annotation was performed by elastic deformation of a template shape, followed by landmark optimization at extreme positions (highest/lowest/most medial/lateral point). The results of our automated annotation method were compared with ground truth, and percentages of landmarks and measurements adhering to different tolerances were calculated. Reliability was evaluated by the intraclass correlation coefficient (ICC). For the manual annotations, the inter-observer absolute difference was 1.53 ± 1.22 mm (mean ± SD) for the landmark positions and 0.56 ± 0.55 mm (mean ± SD) for the morphometric measurements. Automated versus manual landmark extraction differed by an average of 2.05 mm. The automated measurements demonstrated an absolute difference of 0.78 ± 0.60 mm (mean ± SD) from their manual counterparts. Overall, 92% of the automated landmarks were within 4 mm of the expert mean position, and 95% of all morphometric measurements were within 2 mm of the expert mean measurements. The ICC (manual versus automated) for automated morphometric measurements was between 0.926 and 1. Manual annotations required on average 18 min of operator interaction time, while automated annotations only needed 7 min of operator-independent computing time. Considering the time consumption and variability among observers, there is a clear need for a more efficient, standardized, and operator-independent algorithm. Our automated method demonstrated excellent accuracy and reliability for landmark positioning and morphometric measurements. Above all, this automated method will lead to a faster, scalable, and operator-independent morphometric analysis of the knee. DA - 2024/04/11/ PY - 2024 DO - 10.3390/jimaging10040090 DP - DOI.org (Crossref) VL - 10 IS - 4 SP - 90 J2 - J. Imaging LA - en SN - 2313-433X UR - https://www.mdpi.com/2313-433X/10/4/90 AN - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11051533/ DB - PMC Y2 - 2024/05/13/08:27:19 ER - TY - JOUR TI - Synthetic peptides of IL-1Ra and HSP70 have anti-inflammatory activity on human primary monocytes and macrophages: Potential treatments for inflammatory diseases AU - Pensado-López, Alba AU - Ummarino, Aldo AU - Khan, Sophia AU - Guildford, Anna AU - Allan, Iain U. AU - Santin, Matteo AU - Chevallier, Nathalie AU - Varaillon, Elina AU - Kon, Elizaveta AU - Allavena, Paola AU - Torres Andón, Fernando T2 - Nanomedicine: Nanotechnology, Biology and Medicine DA - 2024/01// PY - 2024 DO - 10.1016/j.nano.2023.102719 DP - DOI.org (Crossref) VL - 55 SP - 102719 J2 - Nanomedicine: Nanotechnology, Biology and Medicine LA - en SN - 15499634 ST - Synthetic peptides of IL-1Ra and HSP70 have anti-inflammatory activity on human primary monocytes and macrophages UR - https://linkinghub.elsevier.com/retrieve/pii/S1549963423000709 AN - https://research.brighton.ac.uk/en/publications/synthetic-peptides-of-il-1ra-and-hsp70-have-anti-inflammatory-act DB - University of Brighton Y2 - 2024/05/06/15:50:08 ER - TY - JOUR TI - Stem cells application in meniscal tears: a systematic review of pre-clinical and clinical evidence AU - Rinonapoli, G. AU - Gregori, P. AU - Di Matteo, B. AU - Impieri, L. AU - Ceccarini, P. AU - Manfreda, F. AU - Campofreda, G. AU - Caraffa, A. T2 - European Review for Medical and Pharmacological Sciences AB - OBJECTIVE: Conservative and surgical treatments for meniscal lesions are various and this field of orthopedic surgery is in continuous development. Stem cells represent one of the current options to stimulate meniscal healing. The present systematic review aimed at summarizing the state of art in the application of stem cells for the treatment of meniscal damage both at pre-clinical and clinical level. MATERIALS AND METHODS: The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines were followed to perform this systematic review. A systematic search was performed using the PubMed (MEDLINE), EMBASE and Cochrane Library databases. All the studies dealing with the application of stem cells as a treatment for meniscal tears were pooled, data were extracted and analyzed. The studies were divided into two groups (pre-clinical and clinical), and then, discussed independently. RESULTS: A total of 18 studies were included. Thirteen were classified as “pre-clinical” and five as “clinical”. The most commonly used cells were mesenchymal stem cells (MSC), derived from bone marrow (BMMSC), synovial tissue (SMSC), or adipose tissue (ADSC). Follow-ups ranged from 2 to 16 weeks for the pre-clinical studies and from 3 to 24 months for the clinical studies. All studies documented good results in terms of laboratory markers/scores, clinical and radiologic evaluation. CONCLUSIONS: Based on the currently available data, it is not possible to establish the best cell source or delivery method for the treatment of meniscal injuries. Bone Marrow derived stem cells delivered through injection represent the most studied approach, with the most promising results. However, the full impact of these therapies through their different sub-type of stem cells and implantation techniques still needs to be critically analyzed through larger randomized controlled trials with longer follow-up. DA - 2021/12// PY - 2021 DO - 10.26355/eurrev_202112_27622 DP - DOI.org (CSL JSON) VL - 25 IS - 24 SP - 7754 EP - 7764 LA - eng SN - 1128-3602, 2284-0729 ST - Stem cells application in meniscal tears UR - https://doi.org/10.26355/eurrev_202112_27622 Y2 - 2022/03/17/15:29:06 ER - TY - JOUR TI - Engineering Anisotropic Meniscus: Zonal Functionality and Spatiotemporal Drug Delivery AU - Abbadessa, Anna AU - Crecente-Campo, José AU - Alonso, María José T2 - Tissue Engineering Part B: Reviews DA - 2020/08/26/ PY - 2020 DO - 10.1089/ten.teb.2020.0096 DP - DOI.org (Crossref) J2 - Tissue Engineering Part B: Reviews LA - en SN - 1937-3368, 1937-3376 ST - Engineering Anisotropic Meniscus UR - https://www.liebertpub.com/doi/10.1089/ten.teb.2020.0096 AN - https://minerva.usc.es/xmlui/handle/10347/23900 DB - MINERVA Y2 - 2020/11/30/09:53:45 ER - TY - JOUR TI - Conservative vs. surgical approach for degenerative meniscal injuries: a systematic review of clinical evidence AU - Giuffrida, A. AU - Di Bari, A. AU - Falzone, E. AU - Iacono, F. AU - Kon, E. AU - Marcacci, M. AU - Gatti, R. AU - Di Matteo, B. T2 - European Review for Medical and Pharmacological Sciences AB - OBJECTIVE: Analyzing the available evidence by comparing the role of arthroscopic surgery and conservative treatment in the management of degenerative meniscopathy. MATERIALS AND METHODS: A literature search was carried out on the PubMed, EMBASE, Scopus, and PEDro databases in May 2019 to identify all the randomized controlled trials (RCTs) comparing arthroscopic surgery to conservative management of painful but stable degenerated menisci. The quality of the RCTs was assessed using the Cochrane Risk of Bias Assessment. RESULTS: A total of 10 studies, including 1525 patients and dealing with conservative treatment vs. arthroscopic surgery were included in this review. In eight studies the effectiveness of exercise therapy was compared to surgery; in one study the effectiveness of intra-articular steroid injection was compared to surgery; in one study the effectiveness of placebo surgery was compared to partial meniscectomy. In all studies, no significant inter-group difference in terms of knee pain and knee function were observed at any follow-up evaluation. CONCLUSIONS: Degenerative meniscal tears, without symptoms of locking and catching, can be successfully managed by a proper regimen of physical therapy as a first line treatment. Surgical approach might be considered in case of poor response after conservative treatment. DA - 2020/03// PY - 2020 DO - 10.26355/eurrev_202003_20651 DP - DOI.org (CSL JSON) VL - 24 IS - 6 SP - 2874 EP - 2885 LA - eng SN - 1128-3602, 2284-0729 ST - Conservative vs. surgical approach for degenerative meniscal injuries UR - https://doi.org/10.26355/eurrev_202003_20651 Y2 - 2020/11/19/14:19:33 ER -