@article{korpershoek_selection_2021,
	title = {Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin: A Novel Approach in Meniscus Tissue Engineering},
	volume = {22},
	issn = {1422-0067},
	url = {https://www.mdpi.com/1422-0067/22/16/8614},
	doi = {10.3390/ijms22168614},
	shorttitle = {Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin},
	abstract = {Meniscus injuries can be highly debilitating and lead to knee osteoarthritis. Progenitor cells from the meniscus could be a superior cell type for meniscus repair and tissue-engineering. The purpose of this study is to characterize meniscus progenitor cells isolated by differential adhesion to fibronectin ({FN}-prog). Human osteoarthritic menisci were digested, and {FN}-prog were selected by differential adhesion to fibronectin. Multilineage differentiation, population doubling time, colony formation, and {MSC} surface markers were assessed in the {FN}-prog and the total meniscus population (Men). Colony formation was compared between outer and inner zone meniscus digest. Chondrogenic pellet cultures were performed for redifferentiation. {FN}-prog demonstrated multipotency. The outer zone {FN}-prog formed more colonies than the inner zone {FN}-prog. {FN}-prog displayed more colony formation and a higher proliferation rate than Men. {FN}-prog redifferentiated in pellet culture and mostly adhered to the {MSC} surface marker profile, except for {HLA}-{DR} receptor expression. This is the first study that demonstrates differential adhesion to fibronectin for the isolation of a progenitor-like population from the meniscus. The high proliferation rates and ability to form meniscus extracellular matrix upon redifferentiation, together with the broad availability of osteoarthritis meniscus tissue, make {FN}-prog a promising cell type for clinical translation in meniscus tissue-engineering.},
	pages = {8614},
	number = {16},
	journaltitle = {International Journal of Molecular Sciences},
	shortjournal = {{IJMS}},
	author = {Korpershoek, Jasmijn V. and Rikkers, Margot and de Windt, Tommy S. and Tryfonidou, Marianna A. and Saris, Daniel B. F. and Vonk, Lucienne A.},
	urldate = {2021-10-19},
	date = {2021-08-10},
	langid = {english},
}