@article{korpershoek_selection_2021, title = {Selection of {Highly} {Proliferative} and {Multipotent} {Meniscus} {Progenitors} through {Differential} {Adhesion} to {Fibronectin}: {A} {Novel} {Approach} in {Meniscus} {Tissue} {Engineering}}, volume = {22}, issn = {1422-0067}, shorttitle = {Selection of {Highly} {Proliferative} and {Multipotent} {Meniscus} {Progenitors} through {Differential} {Adhesion} to {Fibronectin}}, url = {https://www.mdpi.com/1422-0067/22/16/8614}, doi = {10.3390/ijms22168614}, abstract = {Meniscus injuries can be highly debilitating and lead to knee osteoarthritis. Progenitor cells from the meniscus could be a superior cell type for meniscus repair and tissue-engineering. The purpose of this study is to characterize meniscus progenitor cells isolated by differential adhesion to fibronectin (FN-prog). Human osteoarthritic menisci were digested, and FN-prog were selected by differential adhesion to fibronectin. Multilineage differentiation, population doubling time, colony formation, and MSC surface markers were assessed in the FN-prog and the total meniscus population (Men). Colony formation was compared between outer and inner zone meniscus digest. Chondrogenic pellet cultures were performed for redifferentiation. FN-prog demonstrated multipotency. The outer zone FN-prog formed more colonies than the inner zone FN-prog. FN-prog displayed more colony formation and a higher proliferation rate than Men. FN-prog redifferentiated in pellet culture and mostly adhered to the MSC surface marker profile, except for HLA-DR receptor expression. This is the first study that demonstrates differential adhesion to fibronectin for the isolation of a progenitor-like population from the meniscus. The high proliferation rates and ability to form meniscus extracellular matrix upon redifferentiation, together with the broad availability of osteoarthritis meniscus tissue, make FN-prog a promising cell type for clinical translation in meniscus tissue-engineering.}, language = {en}, number = {16}, urldate = {2021-10-19}, journal = {International Journal of Molecular Sciences}, author = {Korpershoek, Jasmijn V. and Rikkers, Margot and de Windt, Tommy S. and Tryfonidou, Marianna A. and Saris, Daniel B. F. and Vonk, Lucienne A.}, month = aug, year = {2021}, pages = {8614}, }