@article{korpershoek_potential_2021, title = {Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells}, volume = {22}, issn = {1422-0067}, url = {https://www.mdpi.com/1422-0067/22/20/11200}, doi = {10.3390/ijms222011200}, abstract = {Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting ({MEW}) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling ({FDM}) and with the clinically used Collagen Meniscus Implants® ({CMI}®). The scaffolds were seeded with meniscus and mesenchymal stromal cells ({MSCs}) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in {MEW} scaffolds, the {CMI}®, and fibrin gel control, yet within the {FDM} scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The {MEW} scaffolds showed a higher Young’s modulus when compared to the {CMI}® scaffolds and a higher yield point compared to {FDM} scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with {MEW} using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.}, pages = {11200}, number = {20}, journaltitle = {International Journal of Molecular Sciences}, shortjournal = {{IJMS}}, author = {Korpershoek, Jasmijn V. and Ruijter, Mylène de and Terhaard, Bastiaan F. and Hagmeijer, Michella H. and Saris, Daniël B.F. and Castilho, Miguel and Malda, Jos and Vonk, Lucienne A.}, urldate = {2022-05-30}, date = {2021-10-18}, langid = {english}, }