%0 Journal Article %T Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells %V 22 %N 20 %P 11200 %W https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/34681860/ %U https://www.mdpi.com/1422-0067/22/20/11200 %X Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young’s modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment. %G en %J International Journal of Molecular Sciences %A Korpershoek, Jasmijn V. %A Ruijter, Mylène de %A Terhaard, Bastiaan F. %A Hagmeijer, Michella H. %A Saris, Daniël B.F. %A Castilho, Miguel %A Malda, Jos %A Vonk, Lucienne A. %D 2021-10-18